Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model

Huang, Qun1; Cai, Yuting2,4,5; Yang, Xinrui1,3; Li, Weimin1,3; Pu, Hongji; Liu, Zhenjing4,5; Liu, Hongwei4,5; Tamtaji, Mohsen4,5; Xu, Feng6; Sheng, Liyuan7; Kim, Tae-Hyung8; Zhao, Shiqing9; Sun, Dazhi2; Qin, Jinbao1,3; Luo, Zhengtang4,5; Lu, Xinwu1

2021-12-01

10.1007/s12274-021-3961-3

Nano Research   影响因子和分区

1998-0124

1998-0000

The functional recovery of peripheral nerve injury (PNI) is unsatisfactory, whereas diabetes mellitus (DM) and its related complications further attenuate the restoration of diabetic PNI (DPNI). Adipose-derived stem cells (ADSCs) are promising candidates for treatment of DPNI due to their abundant source, excellent differentiation and paracrine ability. Our results showed that ADSCs remarkably enhanced the proliferation and migration of Schwann cells and endothelial cells, and tube formation. Mechanistically, ADSCs could regulate Nrf2/HO-1, NF-kappa B and PI3K/AKT/mTOR signaling pathways, showing multiple functions in reducing oxidative stress and inflammation, and regulating cell metabolism, growth, survival, proliferation, angiogenesis, differentiation of Schwann cell and myelin formation. In current study, novel graphene foam (GF)/hydrogel-based scaffold was developed to deliver ADSCs for treatment of DPNI. GF/hydrogel scaffold exhibited excellent mechanical strength, suitable porous network, superior electrical conductivity, and good biocompatibility. In vitro results revealed that GF/hydrogel scaffold could obviously accelerate proliferation of Schwann cells. Moreover, in vivo experiments demonstrated that ADSCs-loaded GF/hydrogel scaffold significantly promoted the recovery of DPNI and inhibited the atrophy of targeted muscles, thus providing a novel and attractive therapeutic approach for DPNI patients.

peripheral nerve injury (PNI) diabetes mellitus (DM) adipose-derived stem cells (ADSCs) graphene hydrogel scaffold

SCI ; EI

英语

通讯

National Natural Science Foundation of China[81971758,51890892,81971712,81870346,81700432,11761161004] ; Natural Science Foundation of Shanghai Science and Technology Committee[20ZR1431600] ; National Natural Science Foundationof China-Research Grants Council Joint Research Scheme[11761161004,"N_HKUST607/17"] ; IER foundation[HT-JD-CXY-201907] ; "International science and technology cooperation projects" of Science and Technological Bureau of Guangzhou Huangpu District[2019GH06] ; Guangdong Science and Technology Department[2020A0505090003] ; Research Fund of Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology[2020B1212030010] ; Department of Science and Technology of Guangdong Province[2021B1212040001]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000730512100005

TSINGHUA UNIV PRESS

20215111366575

Biocompatibility ; Cell signaling ; Cytology ; Endothelial cells ; Molecular biology ; Neurons ; Scaffolds (biology) ; Stem cells

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Immunology:461.9.1 ; Nanotechnology:761 ; Chemical Products Generally:804

Web of Science

1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Vasc Surg, Sch Med, Shanghai 200011, Peoples R China
2.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Guangdong Prov Key Lab Funct Oxide Mat & Devices, Shenzhen 518055, Peoples R China
3.Shanghai Jiao Tong Univ, Vasc Ctr, Shanghai 200011, Peoples R China
4.Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Hong Kong 999077, Peoples R China
5.Hong Kong Univ Sci & Technol, William Mong Inst Nano Sci & Technol, Hong Kong 999077, Peoples R China
6.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Bioinspired Engn & Biomech Ctr BEBC, Key Lab Biomed Informat Engn,Minist Educ, Xian 710049, Peoples R China
7.Peking Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China
8.Chung Ang Univ, Sch Integrat Engn, Seoul 06974, South Korea
9.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China

Huang, Qun,Cai, Yuting,Yang, Xinrui,et al. Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model[J]. Nano Research,2021,15:3434-3445.

Huang, Qun.,Cai, Yuting.,Yang, Xinrui.,Li, Weimin.,Pu, Hongji.,...&Lu, Xinwu.(2021).Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model.Nano Research,15,3434-3445.

Huang, Qun,et al."Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model".Nano Research 15(2021):3434-3445.