MiR-155 regulates m6A level and cell progression by targeting FTO in clear cell renal cell carcinoma

Yang，Weifeng1; Xie，Lei1; Wang，Peng2; Zhuang，Changshui1

2022-03-01

10.1016/j.cellsig.2021.110217

CELLULAR SIGNALLING   影响因子和分区

0898-6568

1873-3913

Although FTO, as an eraser of N6-methyladenosine (mA), plays context-dependent tumor-suppressive and oncogenic roles in various cancer type, underlying molecular events of its aberrant expression in cancers is complex and still poorly understood. Here we show that miR-155 directly targets FTO to negatively regulate its expression and increased mA level in ccRCC. Combining bioinformatics analysis and luciferase reporter assays, we identified that miR-155 directly bound to the 3′UTR of FTO mRNA and reduced FTO protein levels in ccRCC cells. Moreover, cell function assays, xenografts assays and mA dot blot assays revealed that overexpression of miR-155 enhanced tumor cell proliferation and global mRNA mA level, while decreasing apoptosis in a FTO-dependent manner. Collectively, our data demonstrates the functional importance of miR-155 in regulating FTO expression and global mRNA mA level, and provides profound insights into ccRCC tumorigenesis.

FTO m6A miR-155 Renal cell carcinoma

SCI

英语

其他

Huazhong Uni-versity of Science and Technology Union Shenzhen Hospital Foundation[YN2021009]

Cell Biology

Cell Biology

WOS:000798298100003

ELSEVIER SCIENCE INC

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85121449412

Scopus

1.Department of Urology,Union Shenzhen Hospital,Huazhong University of Science and Technology,Shenzhen,518052,China
2.Department of Vascular & Interventional Radiology,Southern University of Science and Technology Hospital,Shenzhen,518055,China

Yang，Weifeng,Xie，Lei,Wang，Peng,et al. MiR-155 regulates m6A level and cell progression by targeting FTO in clear cell renal cell carcinoma[J]. CELLULAR SIGNALLING,2022,91.

Yang，Weifeng,Xie，Lei,Wang，Peng,&Zhuang，Changshui.(2022).MiR-155 regulates m6A level and cell progression by targeting FTO in clear cell renal cell carcinoma.CELLULAR SIGNALLING,91.

Yang，Weifeng,et al."MiR-155 regulates m6A level and cell progression by targeting FTO in clear cell renal cell carcinoma".CELLULAR SIGNALLING 91(2022).