Detachable head-mounted photoacoustic microscope in freely moving mice

Heng, Guo1,2; Chen, Qian1,2; Qin, Wei2; Qi, Weizhi2; Xi, Lei1,2

2021-12-15

10.1364/OL.444226

OPTICS LETTERS   影响因子和分区

0146-9592

1539-4794

Optical resolution photoacoustic microscopy (ORPAM) is a promising tool for investigating anatomical and functional dynamics in the cerebral cortex. However, observation in freely moving mice has been a longstanding challenge for ORPAM. In this Letter, we extended ORPAM from anesthetized, head-restrained to awake, freely moving mice by using a detachable head-mounted ORPAM probe. We used a micro-electro-mechanical-system scanner and a miniaturized piezoelectric ultrasonic detector to scan the excitation laser beam and detect generated photoacoustic signals, respectively. The probe weighs 1.8 g and has a large field of view of similar to 3 mm x 3 mm. We evaluated the performance of the probe by carrying out phantom experiments and the imaging of vascular networks in a mouse cerebral cortex. The results suggest that the ORPAM probe is capable of providing stable and high-quality ORPAM images in freely moving mice. (C) 2021 Optica Publishing Group

SCI ; EI

英语

通讯

National Natural Science Foundation of China[61528401,61775028,62022037,81571722] ; Guangdong Science and Technology Department["2019ZT08Y191","SZBL2020090501013"] ; Shenzhen Scientific and Technological Foundation[KQTD20190929172743294] ; Southern University of Science and Technology[PDJH2021C008]

Optics

Optics

WOS:000730864500012

OPTICAL SOC AMER

20220111423316

Laser beams ; Laser excitation ; Mammals ; MEMS ; Photoacoustic microscopy ; Ultrasonic applications

Electric Equipment:704.2 ; Laser Beam Interactions:744.8 ; Laser Applications:744.9 ; Imaging Techniques:746 ; Ultrasonic Applications:753.3 ; Physical Chemistry:801.4

PHYSICS

Web of Science

1.Univ Elect Sci & Technol China, Sch Phys, Chengdu 611731, Sichuan, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China

Heng, Guo,Chen, Qian,Qin, Wei,et al. Detachable head-mounted photoacoustic microscope in freely moving mice[J]. OPTICS LETTERS,2021,46(24):6055-6058.

Heng, Guo,Chen, Qian,Qin, Wei,Qi, Weizhi,&Xi, Lei.(2021).Detachable head-mounted photoacoustic microscope in freely moving mice.OPTICS LETTERS,46(24),6055-6058.

Heng, Guo,et al."Detachable head-mounted photoacoustic microscope in freely moving mice".OPTICS LETTERS 46.24(2021):6055-6058.