南方科技大学知识苑(SUSTech KC): LINC00857 promotes the proliferation of pancreatic cancer via MET, STAT3, and CREB

题名	LINC00857 promotes the proliferation of pancreatic cancer via MET, STAT3, and CREB
作者	Song, Ye 1; Liang, Yingying 1; Zou, Qingfeng 1; Zeng, Shaoting 2; Lin, Hanhong 2; Liu, Meiyuan 1; Liu, Xiaoyan 1; Du, Jike 1; Chen, Guoan3 ; Zou, Lei 4; Su, Wenmei 5; Niu, Feiyu 1
通讯作者	Su, Wenmei; Niu, Feiyu
发表日期	2021-11-01
DOI	10.21037/jgo-21-723
发表期刊	Journal of Gastrointestinal Oncology 影响因子和分区
ISSN	2078-6891
EISSN	2219-679X
卷号	12 期号:6
摘要	Background: Long non-coding RNA (lncRNA) LINC00857 promotes cell proliferation in various cancers and is overexpressed in pancreatic cancer (PC). However, the role of LINC00857 in PC is yet to be clarified. Methods: In this study, we used Gene Expression Profiling Interactive Analysis (GEPIA) to investigate transcriptional data of LINC00857 in different cancers. We determined LINC00857 expression in 4 PC cell lines, and one normal pancreatic cell line by quantitative real-time reverse transcription PCR (qRT-PCR). small interfering RNA (siRNA) was employed to specifically knockdown LINC00857 in BxPc3 and PANC1 cells. Cell proliferation was evaluated using WST-1. Western blotting analysis was used to detect the expression levels of downstream proteins of LINC00857. Results: We revealed that the knockdown of LINC00857 in PC cell lines inhibited the proliferation of the PC cells. We found that LINC00857 downregulation was followed by the downregulation of oncogenic proteins mesenchymal-epithelial transition (MET), signal transducer and activator of transcription 3 (STAT3), and cAMP response element-binding protein (CREB). Conclusions: Our study indicated that LINC00857 regulated the expression of STAT3 and CREB via regulating the expression of MET, and consequently promoted the growth of PC cells. The results allowed us to deepen our understanding of the pathogenesis of PC and provided a potential target for the clinical treatment of PC.
关键词	LINC00857 mesenchymal-epithelial transition (MET) pancreatic cancer proliferation
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	National Natural Science Foundation of China (NSFC)[81702270] ; Clinical Key Specialty Construction Project of Guangzhou Medical University[YYPT202017]
WOS研究方向	Oncology ; Gastroenterology & Hepatology
WOS类目	Oncology ; Gastroenterology & Hepatology
WOS记录号	WOS:000734910100001
出版者	AME PUBL CO
来源库	Web of Science
引用统计	被引频次[WOS]：8
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/259883
专题	南方科技大学医学院
作者单位	1.Guangzhou Med Univ, Affiliated Canc Hosp & Inst, Guangzhou, Peoples R China 2.Guangzhou Med Univ, Guangzhou, Peoples R China 3.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China 4.Kunming Med Univ, Dept Organ Transplant, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China 5.Guangdong Med Univ, Affiliated Hosp, Dept Pulm Oncol, Zhanjiang, Peoples R China
推荐引用方式 GB/T 7714	Song, Ye,Liang, Yingying,Zou, Qingfeng,et al. LINC00857 promotes the proliferation of pancreatic cancer via MET, STAT3, and CREB[J]. Journal of Gastrointestinal Oncology,2021,12(6).
APA	Song, Ye.,Liang, Yingying.,Zou, Qingfeng.,Zeng, Shaoting.,Lin, Hanhong.,...&Niu, Feiyu.(2021).LINC00857 promotes the proliferation of pancreatic cancer via MET, STAT3, and CREB.Journal of Gastrointestinal Oncology,12(6).
MLA	Song, Ye,et al."LINC00857 promotes the proliferation of pancreatic cancer via MET, STAT3, and CREB".Journal of Gastrointestinal Oncology 12.6(2021).