Semiconducting Polymer Dots with Modulated Photoblinking for High-Order Super-Resolution Optical Fluctuation Imaging

Sun, Zezhou1; Liu, Zhihe1; Chen, Haobin2; Li, Rongqin3; Sun, Yujie3; Chen, Danni4; Xu, Gaixia4; Liu, Liwei4; Wu, Changfeng2

2019-05

10.1002/adom.201900007

Advanced Optical Materials   影响因子和分区

2195-1071

Super-resolution optical fluctuation imaging (SOFI) uses photoblinking to achieve fast, background free, 3D fluorescence nanoscopy. The on/off photoblinking characteristics are key factors for realizing high-order SOFI. However, it remains a great challenge to tune the photoblinking of a fluorescent probe because most of the probes are typical single fluorophore systems with stochastic blinking nature. In this study, effective photoblinking modulation is demonstrated in a nanoscale multichromophoric system consisting of donor-acceptor semiconducting polymers. Numerical simulation is performed to quantitatively evaluate the impact of the number of acceptors on the photoblinking of the nanoparticle probe as well as the spatial resolution of the resulting SOFI nanoscopy. In tandem with simulation, a series of polymer dots (Pdots) are designed with varying donor-acceptor moieties that give rise to the modulated photoblinking behaviors, as evidenced by single-particle fluorescence trajectories. The photoblinking behaviors are quantitatively compared in terms of autocorrelation function, dot retention fraction, and on/off time ratio. High-performance photoblinking-based nanoscopy is implemented by using the optimized Pdots, resolving subcellular structure at a spatial resolution of approximate to 95 nm. This study provides a useful approach to engineer the photoblinking properties of fluorescent nanoparticles for super-resolution imaging applications.

fluorescence microscopy photoblinking polymer dots super-resolution optical fluctuation imaging

SCI ; EI

英语

通讯

Shenzhen Science and Technology Innovation Commission[JCYJ20170307110157501]

Materials Science ; Optics

Materials Science, Multidisciplinary ; Optics

WOS:000471340900018

WILEY-V C H VERLAG GMBH

20190806538606

Fluorescence ; Fluorescence microscopy ; Image resolution ; Nanometals ; Nanoparticles ; Optical resolving power ; Optical signal processing ; Probes ; Semiconducting polymers ; Stochastic systems

Semiconducting Materials:712.1 ; Light/Optics:741.1 ; Nanotechnology:761 ; Polymeric Materials:815.1 ; Quantum Theory; Quantum Mechanics:931.4 ; Solid State Physics:933 ; Systems Science:961

Web of Science

1.Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
3.Peking Univ, Sch Life Sci, Biodynam Opt Imaging Ctr BIOPIC, State Key Lab Membrane Biol, Beijing 100871, Peoples R China
4.Shenzhen Univ, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Coll Optoelect Engn, Shenzhen 518060, Peoples R China

Sun, Zezhou,Liu, Zhihe,Chen, Haobin,et al. Semiconducting Polymer Dots with Modulated Photoblinking for High-Order Super-Resolution Optical Fluctuation Imaging[J]. Advanced Optical Materials,2019,7(9).

Sun, Zezhou.,Liu, Zhihe.,Chen, Haobin.,Li, Rongqin.,Sun, Yujie.,...&Wu, Changfeng.(2019).Semiconducting Polymer Dots with Modulated Photoblinking for High-Order Super-Resolution Optical Fluctuation Imaging.Advanced Optical Materials,7(9).

Sun, Zezhou,et al."Semiconducting Polymer Dots with Modulated Photoblinking for High-Order Super-Resolution Optical Fluctuation Imaging".Advanced Optical Materials 7.9(2019).