In situ fabrication of a composite hydrogel with tunable mechanical properties for cartilage tissue engineering

Ma, Fenbo1; Ge, Yongmei1; Liu, Nian1; Pang, Xiangchao1,2; Shen, Xingyu1; Tang, Bin1,3,4

2019-04-21

10.1039/c8tb01331d

Journal of Materials Chemistry B   影响因子和分区

2050-750X

2050-7518

A composite hydrogel with tunable mechanical properties has been fabricated and characterized in this study. We investigated its swelling degree, morphology, structure and thermal stability. Moreover, the effect of strontium chloride concentration on both the dynamic rheology and nanomechanical properties of the composite hydrogels was confirmed in this work. To eliminate the viscoelastic influences of hydrogels during nanomechanical tests, we first analyzed the elastic modulus of strontium alginate (Alg-Sr) and strontium alginate/chondroitin sulfate (Alg/CS-Sr) hydrogels via atomic force microscopy (AFM) using the rate-jump method. Chondrocytes were cultured with the Alg-Sr and Alg/CS-Sr hydrogels respectively. Cell viability assay reveals that the Alg/CS-Sr hydrogel possesses good cytocompatibility. Flow cytometry, qPCR and western blotting analysis suggest that the Alg/CS-Sr hydrogel exerts a positive effect on the inhibition of apoptosis and may exert anti-inflammatory effects in articular cartilage related applications. Furthermore, the preliminary in vivo study shows that the Alg/CS-Sr composite hydrogel facilitates the repair of cartilage in rabbit cartilage defect. Taken together, it is indicated that the Alg/CS-Sr composite hydrogel might be a promising scaffold to promote the repair of cartilage defects.

SCI ; EI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Committee[JCYJ20160517160827379] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170817111312887] ; Shenzhen Science and Technology Innovation Committee[GRCK20160829195523424] ; Shenzhen Science and Technology Innovation Committee[ZDSYS20140509142721429]

Materials Science

Materials Science, Biomaterials

WOS:000465281100005

ROYAL SOC CHEMISTRY

20191606775162

Atomic force microscopy ; Biomechanics ; Cartilage ; Cell death ; Chlorine compounds ; Defects ; Fabrication ; Mechanical properties ; Polymerase chain reaction ; Repair ; Strontium compounds ; Sulfur compounds ; Tissue engineering

Bioengineering and Biology:461 ; Optical Devices and Systems:741.3 ; Biochemistry:801.2 ; Chemical Products Generally:804 ; Maintenance:913.5 ; Materials Science:951

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China
2.Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha, Hunan, Peoples R China
3.Southern Univ Sci & Technol, Guangdong Prov Key Lab Cell Microenvironm & Dis R, 1088 Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Key Lab Cell Microenvironm, 1088 Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China

Ma, Fenbo,Ge, Yongmei,Liu, Nian,et al. In situ fabrication of a composite hydrogel with tunable mechanical properties for cartilage tissue engineering[J]. Journal of Materials Chemistry B,2019,7(15):2463-2473.

Ma, Fenbo,Ge, Yongmei,Liu, Nian,Pang, Xiangchao,Shen, Xingyu,&Tang, Bin.(2019).In situ fabrication of a composite hydrogel with tunable mechanical properties for cartilage tissue engineering.Journal of Materials Chemistry B,7(15),2463-2473.

Ma, Fenbo,et al."In situ fabrication of a composite hydrogel with tunable mechanical properties for cartilage tissue engineering".Journal of Materials Chemistry B 7.15(2019):2463-2473.