New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes

Ma, Liping1,2; Li, Bing3; Zhang, Tong1,4,5

2019-04

10.1016/j.watres.2018.12.069

WATER RESEARCH   影响因子和分区

0043-1354

The proliferation of antibiotic resistance genes (ARGs) in drinking water and their potential horizontal transfer to pathogenic microbes may cause failure of antibiotics. However, antimicrobial resistome monitoring in drinking water is not currently routine. The bacterial hosts of ARGs, especially small-sized microbes in drinking water, may not be effectively removed by membrane filtration disinfection and thus pose threats to human health. In the present study, using metagenomic based approach, we investigated antibiotic resistome of small-sized microbes (0.2-0.45 mu m) in 20 household drinking water samples from 12 cities in Mainland China, Hong Kong and Singapore. A total of 265 ARG subtypes belonging to 17 ARG types were detected at abundances ranging from 4.0 x 10(-2) to 1.0 x 10(0) copies/cell. Multidrug, bacitracin and aminoglycoside resistance genes are dominant, and 43 ARG subtypes were specifically carried by small-size microbes. Metagenomic assembly strategy revealed fragments of three opportunistic pathogen, i.e. Pseudomonas alcaligenes, Pseudomonas aeruginosa and Mycobacterium gordonae, carried mexW, aph(3')-I and aac(2')-I, respectively. Drinking water samples were classified into three groups based on the presence of ARG, pathogen and ARG-carrying pathogen. These new insights into the antibiotic resistome of small microbes in drinking water over a broad scale indicate the need for more comprehensive ARGs monitoring and surveillance of drinking water supplies. These findings, together with the perspectives and strategies proposed in this study, could support initiatives to improve drinking water safety. (C) 2019 Elsevier Ltd. All rights reserved.

Drinking water Public health Antibiotic resistome Pathogenic host Metagenomic sequencing

SCI ; EI

英语

NI期刊

通讯

Shenzhen Knowledge Innovation Program-Basic Research Project[JCYJ20150831192847649]

Engineering ; Environmental Sciences & Ecology ; Water Resources

Engineering, Environmental ; Environmental Sciences ; Water Resources

WOS:000458223900018

PERGAMON-ELSEVIER SCIENCE LTD

20190406406312

Antibiotics ; Bacteria ; Genes ; Health risks ; Microfiltration ; Public health ; Water filtration ; Water supply

Water Resources:444 ; Water Supply Systems:446.1 ; Bioengineering and Biology:461 ; Chemical Operations:802.3

ENVIRONMENT/ECOLOGY

Web of Science

1.Univ Hong Kong, Environm Biotechnol Lab, Pokfulam Rd, Hong Kong, Peoples R China
2.East China Normal Univ, Sch Ecol & Environm Sci, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Shanghai, Peoples R China
3.Tsinghua Univ, Grad Sch Shenzhen, Guangdong Prov Engn Res Ctr Urban Water Recycling, Beijing, Peoples R China
4.Univ Hong Kong, Sch Publ Hlth, Hong Kong, Peoples R China
5.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Int Ctr Antibiot Resistance Environm, Shenzhen, Peoples R China

Ma, Liping,Li, Bing,Zhang, Tong. New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes[J]. WATER RESEARCH,2019,152:191-201.

Ma, Liping,Li, Bing,&Zhang, Tong.(2019).New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes.WATER RESEARCH,152,191-201.

Ma, Liping,et al."New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes".WATER RESEARCH 152(2019):191-201.