A strong, tough, and osteoconductive hydroxyapatite mineralized polyacrylamide/dextran hydrogel for bone tissue regeneration

Fang, Ju1,2; Li, Pengfei3; Lu, Xiong3; Fang, Liming4; Lu, Xiaoying2; Ren, Fuzeng1

2019-04

10.1016/j.actbio.2019.02.019

Acta Biomaterialia   影响因子和分区

1742-7061

1878-7568

The design of hydrogels with adequate mechanical properties and excellent bioactivity, osteoconductivity, and capacity for osseointegration is essential to bone repair and regeneration. However, it is challenging to integrate all these properties into one bone scaffold. Herein, we developed a strong, tough, osteoconductive hydrogel by a facile one-step micellar copolymerization of acrylamide and urethacrylate dextran (Dex-U), followed by the in situ mineralization of hydroxyapatite (HAp) nanocrystals. We show that the soft, flexible, and hydrophobically associated polyacrylamide (PAAm) network is strengthened by the stiff crosslinked Dex-U phase, and that the mineralized HAp simultaneously improves the mechanical properties and osteoconductivity. The obtained HAp mineralized PAAm/Dex-U hydrogel (HAp-PADH) has extremely high compressive strength (6.5 MPa) and enhanced fracture resistance (over 2300 J m(-2)), as compared with pure PAAm hydrogels. In vitro, we show that the mineralized HAp layer promotes the adhesion and proliferation of osteoblasts, and effectively stimulates osteogenic differentiation. Through the in vivo evaluation of hydrogels in a femoral condyle defect rabbit model, we show regeneration of a highly mineralized bone tissue and direct bonding to the HAp-PADH interface. These findings confirm the excellent osteoconductivity and osseointegration ability of fabricated HAp-PADH. The present HAp-PADH, with its superior mechanical properties and excellent osteoconductivity, should have great potential for bone repair and regeneration.

Tough hydrogel Osteoconductive Osseointegration Bone regeneration

SCI ; EI

英语

第一 ; 通讯

Fundamental Research Program of Shenzhen[JCYJ20170307110418960]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000463688900039

ELSEVIER SCI LTD

20215111347952

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
3.Southwest Jiaotong Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Adv Technol Mat, Chengdu 621000, Sichuan, Peoples R China
4.South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China

Fang, Ju,Li, Pengfei,Lu, Xiong,et al. A strong, tough, and osteoconductive hydroxyapatite mineralized polyacrylamide/dextran hydrogel for bone tissue regeneration[J]. Acta Biomaterialia,2019,88:503-513.

Fang, Ju,Li, Pengfei,Lu, Xiong,Fang, Liming,Lu, Xiaoying,&Ren, Fuzeng.(2019).A strong, tough, and osteoconductive hydroxyapatite mineralized polyacrylamide/dextran hydrogel for bone tissue regeneration.Acta Biomaterialia,88,503-513.

Fang, Ju,et al."A strong, tough, and osteoconductive hydroxyapatite mineralized polyacrylamide/dextran hydrogel for bone tissue regeneration".Acta Biomaterialia 88(2019):503-513.