Role of RpoN from Labrenzia aggregata LZB033 (Rhodobacteraceae) in Formation of Flagella and Biofilms, Motility, and Environmental Adaptation

Xu, Tingting1,3,4; Yu, Min1; Liu, Jingli1; Lin, Heyu1; Liang, Jinchang1; Zhang, Xiao-Hua1,2

2019-04

10.1128/AEM.02844-18

APPLIED AND ENVIRONMENTAL MICROBIOLOGY   影响因子和分区

0099-2240

1098-5336

Labrenzia aggregata LZB033 (Rhodobacteraceae), which produces dimeth-ylsulfoniopropionate (DMSP) and reduces nitrate to nitrogen, was isolated from seawater of the East China Sea. Its genome encodes a large number of transcriptional regulators which may be important for its adaptation to diverse marine environments. The alternative sigma(54) factor (RpoN) is a central regulator of many bacteria, regulating the transcription of multiple genes and controlling important cellular functions. However, the exact role of RpoN in Labrenzia spp. is unknown. In this study, an in-frame rpoN deletion mutant was constructed in LZB033, and the function of RpoN was determined. To systematically identify Delta rpoN-controlled genes, we performed a detailed analysis of gene expression differences between the wild-type strain and the Delta rpoN mutant using RNA sequencing. The expression of 175 genes was shown to be controlled by RpoN. Subsequent phenotypic assays showed that the Delta rpoN mutant was attenuated in flagellar biosynthesis and swimming motility, utilized up to 13 carbon substrates differently, lacked the ability to assimilate malic acid, and displayed markedly decreased biofilm formation. In addition, stress response assays showed that the ArpoN mutant was impaired in the ability to survive under different challenge conditions, including osmotic stress, oxidative stress, temperature changes, and acid stress. Moreover, both the DMSP synthesis and catabolism rates of LZB033 decreased after rpoN was knocked out. Our work provides essential insight into the regulatory function of RpoN, revealing that RpoN is a key determinant for LZB033 flagellar formation, motility, biofilm formation, and environmental fitness, as well as DMSP production and degradation.

Labrenzia aggregata RNA sequencing RpoN environmental adaptation in-frame gene knockout

SCI ; EI

英语

其他

National Key Research and Development Program of China[2016YFA0601303]

Biotechnology & Applied Microbiology ; Microbiology

Biotechnology & Applied Microbiology ; Microbiology

WOS:000463406200016

AMER SOC MICROBIOLOGY

20191406740341

Biochemistry ; Biofilms ; Mutagenesis ; RNA

Bioengineering and Biology:461 ; Biomaterials (including synthetics):462.5 ; Biochemistry:801.2

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen Inst Resp Dis, Shenzhen, Peoples R China
4.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen, Peoples R China

Xu, Tingting,Yu, Min,Liu, Jingli,et al. Role of RpoN from Labrenzia aggregata LZB033 (Rhodobacteraceae) in Formation of Flagella and Biofilms, Motility, and Environmental Adaptation[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(7).

Xu, Tingting,Yu, Min,Liu, Jingli,Lin, Heyu,Liang, Jinchang,&Zhang, Xiao-Hua.(2019).Role of RpoN from Labrenzia aggregata LZB033 (Rhodobacteraceae) in Formation of Flagella and Biofilms, Motility, and Environmental Adaptation.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(7).

Xu, Tingting,et al."Role of RpoN from Labrenzia aggregata LZB033 (Rhodobacteraceae) in Formation of Flagella and Biofilms, Motility, and Environmental Adaptation".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.7(2019).