Surgery-free video-oculography in mouse models: enabling quantitative and short-interval longitudinal assessment of vestibular function

Yang, Xiaojie1,2; Zhou, Shiyue1; Wu, Jiaojiao1; Liao, Qun1; Wang, Changquan3; Liu, Minghua1; Qu, Lei1,4; Zhang, Yuan5; Cheng, Cheng5,6,7; Chai, Renjie5,6,8,9; Zhang, Kun1; Yu, Xiaojie10; Huang, Pingbo10,11,12; Liu, Lian13; Xiong, Wei13; Chen, Shi2; Chen, Fangyi1

2019-03-23

10.1016/j.neulet.2018.12.036

NEUROSCIENCE LETTERS   影响因子和分区

0304-3940

1872-7972

Vestibulo-ocular reflex (VOR) responding to acceleration stimuli is originated from the vestibular apparatuses and thus widely used as an in vivo indicator of the vestibular function. We have developed a vestibular function testing (VFT) system that allows to evaluate VOR response with improved efficiency. The previously required surgical procedure has been avoided by using a newly designed animal-immobility setup. The efficacy of our VFT system was demonstrated on the mice with vestibular abnormalities caused by either genetic mutations (Lhfpl5(-/-) or Cdh23(-/)-) or applied vestibulotoxicant (3,3'-iminodipropionitrile, IDPN). Daily longitudinal inspection of the VOR response in the IDPN-administered mice gives the first VOR-based daily-progression profile of the vestibular impairment. The capability of VOR in quantifying the severity of toxicant-induced vestibular deficits has been also demonstrated. The acquired VOR-measurement results were validated against the corresponding behavioral-test results. Further validation against immunofluorescence microscopy was applied to the VOR data obtained from the IDPN-administered mice. We conclude that the improved efficiency of our surgery-free VFT system, firstly, enables the characterization of VOR temporal dynamics and quantification of vestibular-impairment severity that may reveal useful information in toxicological and/or pharmaceutical studies; and, secondly, confers our system promising potential to serve as a high-throughput screener for identifying genes and drugs that affect vestibular function.

Vestibulo-ocular reflex (VOR) Behavioral assay Genetic screen 3,3 '-iminodipropionitrile (IDPN) Ototoxicity Vestibular function Video-oculography (VOG) Mouse model

SCI

英语

第一 ; 通讯

Hong Kong Research Grants Council[GRF16111616] ; Hong Kong Research Grants Council[GRF16102417]

Neurosciences & Neurology

Neurosciences

WOS:000463125800034

ELSEVIER IRELAND LTD

NEUROSCIENCE & BEHAVIOR

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Wuhan Univ, Zhongnan Hosp, Key Lab Combinatorial Biosynth & Drug Discovery, Minist Educ,Sch Pharmaceut Sci, Wuhan 430072, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Microelect, Beijing 100029, Peoples R China
4.Hebei Univ Technol, Dept Biomed Engn, State Key Lab Reliabil & Intelligence Elect Equip, Tianjin 300130, Peoples R China
5.Southeast Univ, Inst Life Sci, Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing 210096, Jiangsu, Peoples R China
6.Res Inst Otolaryngol, Nanjing 210008, Jiangsu, Peoples R China
7.Nanjing Univ, Med Sch, Nanjing Drum Tower Hosp, Dept Otolaryngol Head & Neck Surg, Nanjing 210008, Jiangsu, Peoples R China
8.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226001, Jiangsu, Peoples R China
9.Southeast Univ, Jiangsu Prov High Tech Key Lab Biomed Res, Nanjing 211189, Jiangsu, Peoples R China
10.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China
11.Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Hong Kong, Peoples R China
12.Hong Kong Univ Sci & Technol, State Key Lab Mol Neurosci, Hong Kong, Peoples R China
13.Tsinghua Univ, IDG McGovern Inst Brain Res, Sch Life Sci, Beijing 100084, Peoples R China

Yang, Xiaojie,Zhou, Shiyue,Wu, Jiaojiao,et al. Surgery-free video-oculography in mouse models: enabling quantitative and short-interval longitudinal assessment of vestibular function[J]. NEUROSCIENCE LETTERS,2019,696:212-218.

Yang, Xiaojie.,Zhou, Shiyue.,Wu, Jiaojiao.,Liao, Qun.,Wang, Changquan.,...&Chen, Fangyi.(2019).Surgery-free video-oculography in mouse models: enabling quantitative and short-interval longitudinal assessment of vestibular function.NEUROSCIENCE LETTERS,696,212-218.

Yang, Xiaojie,et al."Surgery-free video-oculography in mouse models: enabling quantitative and short-interval longitudinal assessment of vestibular function".NEUROSCIENCE LETTERS 696(2019):212-218.