Three-Dimensional Printing of Biodegradable Piperazine-Based Polyurethane-Urea Scaffolds with Enhanced Osteogenesis for Bone Regeneration

Ma, Yufei1,3; Hu, Nan4; Liu, Juan1,3; Zhai, Xinyun1,5; Wu, Mingmin1; Hu, Chengshen1; Li, Long2; Lai, Yuxiao2; Pan, Haobo1; Lu, William Weijia5; Zhang, Xinzhou4; Luo, Yanfeng3; Ruan, Changshun1

2019-03-06

10.1021/acsami.8b20323

ACS Applied Materials & Interfaces   影响因子和分区

1944-8244

1944-8252

Synthetic biodegradable polymeric scaffolds with uniformly interconnected pore structure, appropriate mechanical properties, excellent biocompatibility, and even enhanced osteogenesis ability are urgently required for in situ bone regeneration. In this study, for the first time, a series of biodegradable piperazine (PP)-based polyurethane-urea (P-PUU) scaffolds with a gradient of PP contents were developed by air-driven extrusion 3D printing technology. The P-PUU ink of 60 wt % concentration was demonstrated to have appropriate viscosity for scaffold fabrication. The 3D-printed P-PUU scaffolds exhibited an interconnected porous structure of about 450 mu m in macropore size and about 75% in porosity. By regulating the contents of PP in P-PUU scaffolds, their mechanical properties could be moderated, and P-PUU1.4 scaffolds with the highest PP contents exhibited the highest compressive modulus (155.9 +/- 5.7 MPa) and strength (14.8 +/- 1.1 MPa). Moreover, both in vitro and in vivo biological results suggested that the 3D-printed P-PUU scaffolds possessed excellent biocompatibility and osteoconductivity to facilitate new bone formation. The small molecular PP itself was confirmed for the first time to regulate osteogenesis of osteoblasts in a dose-dependent manner and the optimum concentration for osteoconductivity was about similar to 0.5 mM, which suggests that PP molecules, together with the mechanical behavior, nitrogen-contents, and hydrophilicity of P-PUUs, play an important role in enhancing the osteoconductive ability of P-PUU scaffolds. Therefore, the 3D-printed P-PUU scaffolds, with suitable interconnected pore structure, appropriate mechanical properties, and intrinsically osteoconductive ability, should provide a promising alternative for bone regeneration.

synthetic biodegradable polymeric scaffold piperazine-based polyurethane-urea 3D printing bone regeneration

SCI ; EI

英语

其他

Innovation Project for Social Undertaking and Livelihood Security of Chongqing[cstc2017shmsA130087]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000460996900078

AMER CHEMICAL SOC

20190906548975

3D printers ; Biocompatibility ; Biodegradable polymers ; Bone ; Hydrophilicity ; Mechanical properties ; Metabolism ; Polyurethanes ; Pore structure ; Porosity ; Urea

Bioengineering and Biology:461 ; Printing Equipment:745.1.1 ; Organic Compounds:804.1 ; Polymeric Materials:815.1 ; Organic Polymers:815.1.1 ; Physical Properties of Gases, Liquids and Solids:931.2 ; Materials Science:951

Web of Science

1.Chinese Acad Sci, Inst Biomed & Biotechnol, Res Ctr Human Tissue & Organs Degenerat, Shenzhen 518055, Guangdong, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Translat Med Res & Dev Ctr, Shenzhen 518055, Guangdong, Peoples R China
3.Chongqing Univ, Coll Bioengn, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China
4.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp,Dept Nephrol, Affiliated Hosp 1,Clin Med Coll 2,Key Lab Shenzhe, Shenzhen 518020, Guangdong, Peoples R China
5.Univ Hong Kong, Dept Orthopaed & Traumatol, Pokfulam, 21 Sassoon Rd, Hong Kong 999077, Peoples R China

Ma, Yufei,Hu, Nan,Liu, Juan,et al. Three-Dimensional Printing of Biodegradable Piperazine-Based Polyurethane-Urea Scaffolds with Enhanced Osteogenesis for Bone Regeneration[J]. ACS Applied Materials & Interfaces,2019,11(9):9415-9424.

Ma, Yufei.,Hu, Nan.,Liu, Juan.,Zhai, Xinyun.,Wu, Mingmin.,...&Ruan, Changshun.(2019).Three-Dimensional Printing of Biodegradable Piperazine-Based Polyurethane-Urea Scaffolds with Enhanced Osteogenesis for Bone Regeneration.ACS Applied Materials & Interfaces,11(9),9415-9424.

Ma, Yufei,et al."Three-Dimensional Printing of Biodegradable Piperazine-Based Polyurethane-Urea Scaffolds with Enhanced Osteogenesis for Bone Regeneration".ACS Applied Materials & Interfaces 11.9(2019):9415-9424.