Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function

Fang, Qiaojun1,2,5; Zhang, Yuhua2; Da, Peng3; Shao, Buwei2; Pan, Haolai4,11,12; He, Zuhong2,6; Cheng, Cheng2; Li, Dan2; Guo, Jiaqi2; Wu, Xiaohan2; Guan, Ming13; Liao, Menghui2; Zhang, Yuan2; Sha, Suhua5; Zhou, Zikai2; Wang, Jian4,7; Wang, Tian14; Su, Kaiming4; Chai, Renjie2,8,9,10; Chen, Fangyi1

2019-03-04

10.1038/s41598-019-39769-z

Scientific Reports   影响因子和分区

2045-2322

Inherited hearing loss is associated with gene mutations that result in sensory hair cell (HC) malfunction. HC structure is defined by the cytoskeleton, which is mainly composed of actin filaments and actin-binding partners. LIM motif-containing protein kinases (LIMKs) are the primary regulators of actin dynamics and consist of two members: LIMK1 and LIMK2. Actin arrangement is directly involved in the regulation of cytoskeletal structure and the maturation of synapses in the central nervous system, and LIMKs are involved in structural plasticity by controlling the activation of the actin depolymerization protein cofilin in the olfactory system and in the hippocampus. However, the expression pattern and the role of LIMKs in mouse cochlear development and synapse function also need to be further studied. We show here that the Limk genes are expressed in the mouse cochlea. We examined the morphology and the afferent synapse densities of HCs and measured the auditory function in Limk1 and Limk2 double knockout (DKO) mice. We found that the loss of Limk1 and Limk2 did not appear to affect the overall development of the cochlea, including the number of HCs and the structure of hair bundles. There were no significant differences in auditory thresholds between DKO mice and wild-type littermates. However, the expression of p-cofilin in the DKO mice was significantly decreased. Additionally, no significant differences were found in the number or distribution of ribbon synapses between the DKO and wild-type mice. In summary, our data suggest that the Limk genes play a different role in the development of the cochlea compared to their role in the central nervous system.

SCI

英语

第一 ; 通讯

Fundamental Research Funds for the Central Universities[2242018k1G011]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000460123600029

NATURE PUBLISHING GROUP

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518000, Peoples R China
2.Southeast Univ, Inst Life Sci, Key Lab Dev Genes & Human Dis, Minist Educ, Nanjing 210096, Jiangsu, Peoples R China
3.Nantong Univ, Dept Otolaryngol Head & Neck Surg, Affiliated Hosp, Nantong 226001, Peoples R China
4.Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Otolaryngol, 600 Yishan Rd, Shanghai 200233, Peoples R China
5.Med Univ South Carolina, Dept Pathol & Lab Med, Charleston, SC 29425 USA
6.Huazhong Univ Sci & Technol, Union Hosp, Dept Otorhinolaryngol, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China
7.Dalhousie Univ, Sch Human Commun Disorders, Halifax, NS B3J1Y6, Canada
8.Southeast Univ, Jiangsu Prov High Tech Key Lab Biomed Res, Nanjing 211189, Jiangsu, Peoples R China
9.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226001, Peoples R China
10.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China
11.Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 112008, Peoples R China
12.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 112008, Peoples R China
13.Hangzhou First Peoples Hosp, Dept Otolaryngol, Hangzhou 310006, Zhejiang, Peoples R China
14.Cent S Univ, Xiangya Hosp 2, Dept Otolaryngol Head & Neck Surg, Changsha 410011, Hunan, Peoples R China

Fang, Qiaojun,Zhang, Yuhua,Da, Peng,et al. Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function[J]. Scientific Reports,2019,9.

Fang, Qiaojun.,Zhang, Yuhua.,Da, Peng.,Shao, Buwei.,Pan, Haolai.,...&Chen, Fangyi.(2019).Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function.Scientific Reports,9.

Fang, Qiaojun,et al."Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function".Scientific Reports 9(2019).