Live-cell imaging of octaarginine-modified polymer dots via single particle tracking

Luo, Yao1,2,3; Han, Yuping2,3,4; Hu, Xingjie2,3,5; Yin, Min2,3,6; Wu, Changfeng7; Li, Qian2,3; Chen, Nan2,3,6; Zhao, Yun1

2019-03

10.1111/cpr.12556

CELL PROLIFERATION   影响因子和分区

0960-7722

1365-2184

Objectives Nanocarriers can greatly enhance the cellular uptake of therapeutic agents to regulate cell proliferation and metabolism. Nevertheless, further application of nanocarriers is often limited by insufficient understanding of the mechanisms of their uptake and intracellular behaviour. Materials and methods Fluorescent polymer dots (Pdots) are coated with synthetic octaarginine peptides (R8) and are analysed for cellular uptake and intracellular transportation in HeLa cervical cancer cells via single particle tracking. Results Surface modification with the R8 peptide efficiently improves both cellular uptake and endosomal escape of Pdots. With single particle tracking, we capture the dynamic process of internalization and intracellular trafficking of R8-Pdots, providing new insights into the mechanism of R8 in facilitating nanostructure-based cellular delivery. Furthermore, our results reveal R8-Pdots as a novel type of autophagy inducer. Conclusions This study provides new insights into R8-mediated cellular uptake and endosomal escape of nanocarriers. It potentiates biological applications of Pdots in targeted cell imaging, drug delivery and gene regulation.

endosomal escape live-cell imaging octaarginine peptide polymer dots single particle tracking

SCI

英语

其他

Key Research Program of Frontier Sciences[QYZDJ-SSW-SLH019] ; Key Research Program of Frontier Sciences[QYZDJ-SSW-SLH031]

Cell Biology

Cell Biology

WOS:000463046500006

WILEY

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Sichuan Univ, Coll Life Sci, Chengdu, Sichuan, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai, Peoples R China
4.Chengdu Med Coll, Dept Anat & Histol & Embryol, Dev & Regenerat Key Lab Sichuan Prov, Chengdu, Sichuan, Peoples R China
5.Guangzhou Med Univ, Sch Publ Hlth, Guangzhou, Guangdong, Peoples R China
6.Shanghai Normal Univ, Dept Chem, Shanghai, Peoples R China
7.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Peoples R China

Luo, Yao,Han, Yuping,Hu, Xingjie,et al. Live-cell imaging of octaarginine-modified polymer dots via single particle tracking[J]. CELL PROLIFERATION,2019,52(2).

Luo, Yao.,Han, Yuping.,Hu, Xingjie.,Yin, Min.,Wu, Changfeng.,...&Zhao, Yun.(2019).Live-cell imaging of octaarginine-modified polymer dots via single particle tracking.CELL PROLIFERATION,52(2).

Luo, Yao,et al."Live-cell imaging of octaarginine-modified polymer dots via single particle tracking".CELL PROLIFERATION 52.2(2019).