TSA restores hair follicle-inductive capacity of skin-derived precursors

Guo, Ling1,2,4,5; Wang, Xiaoxiao1,2,3,4,5; Yuan, Jifan4,5; Zhu, Meishu6; Fu, Xiaobing7,8; Xu, Ren-He9,10; Wu, Chuanyue11; Wu, Yaojiong1,2,3

2019-02-27

10.1038/s41598-019-39394-w

Scientific Reports   影响因子和分区

2045-2322

The genesis of the hair follicle relies on signals derived from mesenchymal cells in the dermis during skin morphogenesis and regeneration. Multipotent skin-derived precursors (SKPs), which exhibit long term proliferation potential when being cultured in spheroids, have been shown to induce hair genesis and hair follicle regeneration in mice, implying a therapeutic potential of SKPs in hair follicle regeneration and bioengineering. However, the hair-inductive property of SKPs declines progressively upon ex vivo culture expansion, suggesting that the expressions of the genes responsible for hair induction are epigenetically unstable. In this study, we found that TSA markedly alleviated culture expansion induced SKP senescence, increased the expression and activity of alkaline phosphatase (AP) in the cells and importantly restored the hair inductive capacity of SKPs. TSA increased the acetylation level of histone H3, including the K19/14 sites in the promoter regions of bone morphogenetic proteins (BMPs) genes, which were associated with elevated gene expression and BMP signaling activity, suggesting a potential attribution of BMP pathway in TSA induced recovery of the hair inductive capacity of SKPs.

SCI

英语

其他

Shenzhen Science and Technology Innovation Committee[JCY20160301150838144] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170818093935581] ; Shenzhen Science and Technology Innovation Committee[JCYJ20150831142427959] ; Shenzhen Science and Technology Innovation Committee[ZDSYS20140509142721429]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000459799800016

NATURE PUBLISHING GROUP

Web of Science

1.Tsinghua Univ, Grad Sch Shenzhen, State Key Lab Chem Oncogen, Shenzhen, Peoples R China
2.Tsinghua Univ, Grad Sch Shenzhen, Shenzhen Key Lab Hlth Sci & Technol, Shenzhen, Peoples R China
3.Tsinghua Univ, TBSI, Shenzhen, Peoples R China
4.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen Key Lab Cell Microenvironm, Shenzhen, Peoples R China
5.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Dept Biol, Shenzhen, Peoples R China
6.Shenzhen Univ, Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen, Peoples R China
7.Chinese Peoples Liberat Army Gen Hosp, Inst Basic Med Sci, Wound Healing & Cell Biol Lab, Beijing, Peoples R China
8.Gen Hosp PLA, Affiliated Hosp 1, Stem Cell & Tissue Regenerat Lab, Beijing, Peoples R China
9.Univ Macau, Inst Translat Med, Taipa, Macao, Peoples R China
10.Ctr Reprod Dev & Aging, Fac Hlth Sci, Taipa, Macao, Peoples R China
11.Univ Pittsburgh, Dept Pathol, Sch Med, Pittsburgh, PA 15261 USA

Guo, Ling,Wang, Xiaoxiao,Yuan, Jifan,et al. TSA restores hair follicle-inductive capacity of skin-derived precursors[J]. Scientific Reports,2019,9.

Guo, Ling.,Wang, Xiaoxiao.,Yuan, Jifan.,Zhu, Meishu.,Fu, Xiaobing.,...&Wu, Yaojiong.(2019).TSA restores hair follicle-inductive capacity of skin-derived precursors.Scientific Reports,9.

Guo, Ling,et al."TSA restores hair follicle-inductive capacity of skin-derived precursors".Scientific Reports 9(2019).