Alginate/chondroitin sulfate based hybrid hydrogel with different molecular weight and its capacity to regulate chondrocytes activity

Ma Fenbo1; Pang Xiangchao1,2; Tang Bin1,3,4

2019-02-15

10.1016/j.carbpol.2018.10.109

CARBOHYDRATE POLYMERS   影响因子和分区

0144-8617

1879-1344

Chondrocytes culture within three-dimensional (3D) hydrogels is an attractive strategy for expanding their potential in various biomedical applications. Molecular weight (MW) of the polymers used for preparing one-component hydrogels for 3D cell culture can affect the activity of chondrocytes. However, whether MW of double network hydrogels affects cell fate remains to be determined. Here, a ternary hydrogel was employed to encapsulate chondrocytes. By altering MW of alginate, the hybrid hydrogels with different mechanical properties were prepared. The effects of MW on the properties of hydrogels were evaluated in this work. Cell results revealed that chondrocytes encapsulated in low-MW hydrogels exhibited the best results on maintaining cell viability and inhibiting cell death. Additionally, RT-qPCR analysis suggested that the expression of COLII was increased while the expression of IL-1 beta and iNOS was decreased in chondrocytes encapsulated in low-MW hydrogels. Hence, it is reasonable to be expected that the developed low-MW hydrogel should be a promising candidate for cartilage tissue engineering.

Hydrogel Molecular weight Biocompatible 3D culture

SCI ; EI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Committee[JCYJ20160517160827379] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170817111312887] ; Shenzhen Science and Technology Innovation Committee[GRCK20160829195523424] ; Shenzhen Science and Technology Innovation Committee[ZDSYS20140509142721429]

Chemistry ; Polymer Science

Chemistry, Applied ; Chemistry, Organic ; Polymer Science

WOS:000452982700026

ELSEVIER SCI LTD

20184606083234

Biocompatibility ; Biomechanics ; Cell culture ; Cell death ; Medical applications ; Molecular weight ; Polymerase chain reaction ; Sulfur compounds ; Tissue engineering

Bioengineering and Biology:461 ; Biochemistry:801.2 ; Chemical Products Generally:804 ; Atomic and Molecular Physics:931.3

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China
2.Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha, Hunan, Peoples R China
3.Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen, Peoples R China
4.Shenzhen Key Lab Cell Microenvironm, Shenzhen, Peoples R China

Ma Fenbo,Pang Xiangchao,Tang Bin. Alginate/chondroitin sulfate based hybrid hydrogel with different molecular weight and its capacity to regulate chondrocytes activity[J]. CARBOHYDRATE POLYMERS,2019,206:229-237.

Ma Fenbo,Pang Xiangchao,&Tang Bin.(2019).Alginate/chondroitin sulfate based hybrid hydrogel with different molecular weight and its capacity to regulate chondrocytes activity.CARBOHYDRATE POLYMERS,206,229-237.

Ma Fenbo,et al."Alginate/chondroitin sulfate based hybrid hydrogel with different molecular weight and its capacity to regulate chondrocytes activity".CARBOHYDRATE POLYMERS 206(2019):229-237.