Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii

Wang, Jing1,2; Liu, Xi1,3; Yu, Kaiwei1; Liu, Moxiao1; Qu, Jiuxin2; Liu, Yingxia2; Cai, Zhao1; Wang, Ke4; Zhuo, Chao5; Yang, Liang1,6; Zhang, Yingdan1,6

2021-12-01

10.1021/accinfecdis.1c00416

ACS Infectious Diseases   影响因子和分区

2373-8227

Co-infection of Pscudomonas acruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate. Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes. Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biolilm model. Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress. We further found other EPS structural liber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment. Thus, targeting the EPS structural fibers such as Psi and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.

dual-species biofilms interspecies interaction antibiotic resistance EPS

SCI

英语

第一 ; 通讯

Guangdong Natural Science Foundation for Distinguished Young Scholars[2020B1515020003] ; Shenzhen Key Laboratory of Gene Regulation and Systems Biology, Southern University of Science and Technology[ZDSYS20200811144002008] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515110640] ; China Postdoctoral Science Foundation[2020M672649] ; Guangzhou Municipal Science and Technology Bureau[201607020044]

Pharmacology & Pharmacy ; Infectious Diseases

Chemistry, Medicinal ; Infectious Diseases

WOS:000736563200001

AMER CHEMICAL SOC

Web of Science

1.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen 518112, Peoples R China
3.South China Agr Univ, Integrat Microbiol Res Ctr, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou 510642, Peoples R China
4.GuangXi Med Univ, Affiliated Hosp 1, Nanning 530021, Guangxi, Peoples R China
5.Guangzhou Med Univ, Affiliated Hosp 1, State Key Lab Resp Dis, Guangzhou 510120, Guangdong, Peoples R China
6.Southern Univ Sci & Technol, Shenzhen Key Lab Gene Regulat & Syst Biol, Shenzhen 518055, Peoples R China

Wang, Jing,Liu, Xi,Yu, Kaiwei,et al. Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii[J]. ACS Infectious Diseases,2021,8(1).

Wang, Jing.,Liu, Xi.,Yu, Kaiwei.,Liu, Moxiao.,Qu, Jiuxin.,...&Zhang, Yingdan.(2021).Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.ACS Infectious Diseases,8(1).

Wang, Jing,et al."Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii".ACS Infectious Diseases 8.1(2021).