A preliminary analysis of mitochondrial DNA atlas in the type 2 diabetes patients

Li, Chunhong1,2,3; Xiang, Yueying4; Zhang, Yanyan4,5; Tang, Donge1,2; Chen, Yan2; Xue, Wen2; Wang, Xiaobin4; Chen, Jiejing2; Dai, Yong1,2

2022

10.1007/s13410-021-01031-6

International Journal of Diabetes in Developing Countries   影响因子和分区

0973-3930

1998-3832

Background Type 2 diabetes (T2D), one of the most common metabolic diseases, is the result of insulin resistance or impaired insulin secretion due to mitochondrial dysfunction. Mitochondrial DNA (mtDNA) polymorphisms play an important role in the physiological and pathological characteristics of T2D. However, their mechanism is poorly understood. Methods To directly identify candidate mtDNA variants associated with T2D at the genome-wide level, we constructed forty libraries from ten patients with T2D and thirty control individuals for deep sequencing. We characterized their mtDNA atlas, analysed their single nucleotide polymorphisms (MtSNPs) and screened potential mtDNA mutation sites associated with T2D. Results We found ten mtDNA polymorphisms at nucleotides 489 T > C, 3105AC > A, 3107 N > C, 8701A > G, 9540 T > C, 10398A > G, 10400C > T, 10873 T > C, 12705C > T and 14783 T > C that showed a significant difference between patients and control subjects. Conclusion Therefore, our results characterize the mtDNA atlas of patients with T2D and further demonstrate that mtDNA variants participate in the pathophysiology of T2D and other diseases. In addition, mtDNA variants may be candidate molecular biomarkers of T2D and may be valuable for early diagnosis of T2D in the future.

Type 2 diabetes Mitochondrial DNA Single nucleotide polymorphisms Molecular biomarkers

SCI

英语

第一 ; 通讯

Natural Science Foundation of Shenzhen University General Hospital[857060000040557]

Endocrinology & Metabolism

Endocrinology & Metabolism

WOS:000737094000001

SPRINGER INDIA

Web of Science

1.Southern Univ Sci & Technol, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Shenzhen Engn Res Ctr Autoimmune Dis,Affiliated H, Clin Med Res Ctr,Shenzhen Peoples Hosp,Clin Med C, Shenzhen 518020, Peoples R China
2.Guilin 924 Hosp, Cent Lab, Guangxi Key Lab Metab Dis Res, Guilin 541002, Peoples R China
3.Guilin Med Univ, Cent Lab, Guangxi Hlth Commiss Key Lab Glucose & Lipid Meta, Affiliated Hosp 2, Guilin 541199, Guangxi, Peoples R China
4.Shenzhen Univ Gen Hosp, Shenzhen Univ Clin Med Acad, Shenzhen 518055, Peoples R China
5.Shenzhen Univ, Dept Basic Med, Hlth Sci Ctr, Shenzhen 518060, Peoples R China

Li, Chunhong,Xiang, Yueying,Zhang, Yanyan,et al. A preliminary analysis of mitochondrial DNA atlas in the type 2 diabetes patients[J]. International Journal of Diabetes in Developing Countries,2022.

Li, Chunhong.,Xiang, Yueying.,Zhang, Yanyan.,Tang, Donge.,Chen, Yan.,...&Dai, Yong.(2022).A preliminary analysis of mitochondrial DNA atlas in the type 2 diabetes patients.International Journal of Diabetes in Developing Countries.

Li, Chunhong,et al."A preliminary analysis of mitochondrial DNA atlas in the type 2 diabetes patients".International Journal of Diabetes in Developing Countries (2022).