Sequential immunization with SARS-CoV-2 RBD vaccine induces potent and broad neutralization against variants in mice

Song，Shuo1,2; Zhou，Bing1,2; Cheng，Lin1,2; Liu，Weilong1,2; Fan，Qing1,2; Ge，Xiangyang1,2; Peng，Hua5,6; Fu，Yang Xin7; Ju，Bin1,2,4; Zhang，Zheng1,2,3,4

2022-12-01

10.1186/s12985-021-01737-3

Virology Journal   影响因子和分区

1743-422X

The current COVID-19 pandemic caused by constantly emerging SARS-CoV-2 variants still poses a threat to public health worldwide. Effective next-generation vaccines and optimized booster vaccination strategies are urgently needed. Here, we sequentially immunized mice with a SARS-CoV-2 wild-type inactivated vaccine and a heterologous mutant RBD vaccine, and then evaluated their neutralizing antibody responses against variants including Beta, Delta, Alpha, Iota, Kappa, and A.23.1. These data showed that a third booster dose of heterologous RBD vaccine especially after two doses of inactivated vaccines significantly enhanced the GMTs of nAbs against all SARS-CoV-2 variants we tested. In addition, the WT and variants all displayed good cross-immunogenicity and might be applied in the design of booster vaccines to induce broadly neutralizing antibodies.

Broadly neutralizing antibody Inactivated vaccine Mutant RBD vaccine SARS-CoV-2 variant Sequential immunization

SCI

英语

通讯

WOS:000738608100001

2-s2.0-85122330427

Scopus

1.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People’s Hospital,Shenzhen,Guangdong Province,China
2.The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong Province,China
3.Shenzhen Research Center for Communicable Disease Diagnosis and Treatment of Chinese Academy of Medical Science,Shenzhen,Guangdong Province,China
4.Guangdong Key Laboratory for Anti-Infection Drug Quality Evaluation,Shenzhen,Guangdong Province,China
5.Institute of Biophysics,Chinese Academy of Sciences,Beijing,China
6.Guangzhou Laboratory,and Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou,China
7.Department of Basic Medical Sciences,School of Medicine,Tsinghua University,Beijing,China

Song，Shuo,Zhou，Bing,Cheng，Lin,et al. Sequential immunization with SARS-CoV-2 RBD vaccine induces potent and broad neutralization against variants in mice[J]. Virology Journal,2022,19(1).

Song，Shuo.,Zhou，Bing.,Cheng，Lin.,Liu，Weilong.,Fan，Qing.,...&Zhang，Zheng.(2022).Sequential immunization with SARS-CoV-2 RBD vaccine induces potent and broad neutralization against variants in mice.Virology Journal,19(1).

Song，Shuo,et al."Sequential immunization with SARS-CoV-2 RBD vaccine induces potent and broad neutralization against variants in mice".Virology Journal 19.1(2022).