南方科技大学知识苑(SUSTech KC): A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis

题名	A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis
作者	Zhou, Hua1 ; Zhu, Wei1 ; Wang, Xuncheng 2; Bian, Yeting 3; Jiang, Yan1 ; Li, Jian1 ; Wang, Lixia 4; Yin, Ping 4; Deng, Xing Wang 1,5,6; Xu, Dongqing 3
通讯作者	Deng, Xing Wang; Xu, Dongqing
发表日期	2022
DOI	10.1111/nph.17932
发表期刊	NEW PHYTOLOGIST 影响因子和分区
ISSN	0028-646X
EISSN	1469-8137
摘要	CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) mediates various cellular and physiological processes in plants by targeting a large number of substrates for ubiquitination and degradation. In this study, we reveal that a substitution of Pro for Leu at amino acid position 409 in WRKY32 largely suppresses the short hypocotyls and expanded cotyledon phenotypes of cop1-6. WRKY32(P409L) promotes hypocotyl growth and inhibits the opening of cotyledons in Arabidopsis. Loss of WRKY32 function mutant seedlings display elongated hypocotyls, whereas overexpression of WRKY32 leads to shortened hypocotyls. WRKY32 directly associates with the promoter regions of HY5 to activate its transcription. COP1 interacts with and targets WRKY32 for ubiquitination and degradation in darkness. WRKY32(P409L) exhibits enhanced DNA binding ability and affects the expression of more genes compared with WRKY32 in Arabidopsis. Our results not only reveal the basic role for WRKY32 in promoting photomorphogenesis, but also provide insights into manipulating plant growth by engineering key components of light signaling.
关键词	Arabidopsis COP1 HY5 light signaling photomorphogenesis WRKY32
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Key R&D Program of China[2017YFA0503800] ; National Natural Science Foundation of China[31970258,31900210,31621001] ; Jiangsu Natural Science Foundation for Distinguished Young Scholars[BK20211525]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000739911400001
出版者	WILEY
ESI学科分类	PLANT & ANIMAL SCIENCE
来源库	Web of Science
引用统计	被引频次[WOS]：10
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/264413
专题	南方科技大学-北京大学植物与食品联合研究所生命科学学院生命科学学院_生物系
作者单位	1.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory, Inst Plant & Food Sci, Sch Life Sci,Guangdong Higher Educ Inst,Dept Biol, Shenzhen 518055, Peoples R China 2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing 100097, Peoples R China 3.Nanjing Agr Univ, Coll Agr, Natl Ctr Soybean Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China 4.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan 430070, Peoples R China 5.Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China 6.Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China
第一作者单位	南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院
通讯作者单位	南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院
第一作者的第一单位	南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院
推荐引用方式 GB/T 7714	Zhou, Hua,Zhu, Wei,Wang, Xuncheng,et al. A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis[J]. NEW PHYTOLOGIST,2022.
APA	Zhou, Hua.,Zhu, Wei.,Wang, Xuncheng.,Bian, Yeting.,Jiang, Yan.,...&Xu, Dongqing.(2022).A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis.NEW PHYTOLOGIST.
MLA	Zhou, Hua,et al."A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis".NEW PHYTOLOGIST (2022).