Molecular Design of -Sheet Peptide for the Multi-Modal Analysis of Disease

Cao, Hongyan1,2,3; Wang, Yunyun1,2,3; Gao, Yuan1,2; Deng, Xinli3; Cong, Yulong3; Liu, Ye1,2,4,6,7; Jiang, Xingyu1,2,4,5

2019-02-04

10.1002/anie.201809716

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

Intermolecular forces constrain peptide conformation. However, the role of each intermolecular force in constraining peptide conformation remains poorly understood. In this work, we show that aromatic-aromatic interactions drive peptides into -sheets, and the hydrophobic effect determines the assembly speed of peptides. By using intermolecular forces to artificially control the assembly of -sheets, a multi-modal analytical system was developed that allows five readouts and dual qualitative-quantitative analysis, and satisfies both point-of-care testing (POCT) and laboratory-based testing. For Mycoplasma Pneumoniae diagnosis, this system eradicates misdiagnosis (from 30% to 0%) and broadens the linear range by three-fold, both of which are critical for guiding therapy. This work not only illustrates exact roles of intermolecular forces in driving the formation of -sheets, but also provides a guideline for the construction of a multi-modal analytical system for disease diagnosis.

conformationally constrained peptide intermolecular force multi-modal analysis mycoplasma pneumoniae diagnosis

SCI ; EI ; IC

英语

NI期刊

通讯

Chinese Academy of Sciences[XDA09030305]

Chemistry

Chemistry, Multidisciplinary

WOS:000458826800011

WILEY-V C H VERLAG GMBH

20190306377752

Aromatic compounds ; Chemical bonds ; Diagnosis ; Hydrophobicity ; Molecules ; Peptides

Medicine and Pharmacology:461.6 ; Biology:461.9 ; Physical Chemistry:801.4 ; Organic Compounds:804.1 ; Mathematics:921 ; Physical Properties of Gases, Liquids and Solids:931.2 ; Atomic and Molecular Physics:931.3

CHEMISTRY

Web of Science

1.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China
2.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China
3.Chinese Peoples Liberat Army Gen Hosp, Clin Lab South Bldg, Beijing 100853, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
6.Chinese Acad Med Sci, Inst Med Biol, Kunming 650000, Yunnan, Peoples R China
7.Peking Union Med Coll, Kunming 650000, Yunnan, Peoples R China

Cao, Hongyan,Wang, Yunyun,Gao, Yuan,et al. Molecular Design of -Sheet Peptide for the Multi-Modal Analysis of Disease[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2019,58(6):1626-1631.

Cao, Hongyan.,Wang, Yunyun.,Gao, Yuan.,Deng, Xinli.,Cong, Yulong.,...&Jiang, Xingyu.(2019).Molecular Design of -Sheet Peptide for the Multi-Modal Analysis of Disease.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,58(6),1626-1631.

Cao, Hongyan,et al."Molecular Design of -Sheet Peptide for the Multi-Modal Analysis of Disease".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 58.6(2019):1626-1631.