The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition

Wang, Hongzhong1; Yu, Junyan1; Wang, Xingshun1,2; Zhang, Yandong1

2019-02

10.1016/j.cellsig.2018.12.005

CELLULAR SIGNALLING   影响因子和分区

0898-6568

1873-3913

Human Glioblastoma is one deadly disease; the median survival time is reported to be 13.9 months after treatment. In the present study, we discovered that DHX33 is highly expressed in 84% of all Glioblastoma multiforme (GBM). Knockdown of DHX33 led to significant reduced proliferation and migration in glioblastoma cells in vitro and in vivo. Mechanistically, DHX33 regulated a set of critical genes involved in cell cycle and cell migration to promote glioblastoma development. Additionally, DHX33 was found to be induced by inhibitors of PI3K and mTOR whose activation has been detected in 50% of glioblastoma. Overexpression of wild type DHX33 protein, but not the helicase dead mutant, confers resistance to mTOR inhibitors in glioblastoma cells. DHX33 probably functions as a critical regulator to promote GBM development. Our results highlight its therapeutic potential in treating GBM.

DHX33 RNA helicase PI3K/Akt/mTOR Glioblastoma

SCI

英语

第一 ; 通讯

Guangdong Provincial Science and Technology Department[2017A020211024]

Cell Biology

Cell Biology

WOS:000456752900018

ELSEVIER SCIENCE INC

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Southern Univ Sci & Technol, Dept Biol, 1088 Xueyuan Blvd, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Univ Macau Joint PhD Program, Shenzhen, Guangdong, Peoples R China

Wang, Hongzhong,Yu, Junyan,Wang, Xingshun,et al. The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition[J]. CELLULAR SIGNALLING,2019,54:170-178.

Wang, Hongzhong,Yu, Junyan,Wang, Xingshun,&Zhang, Yandong.(2019).The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.CELLULAR SIGNALLING,54,170-178.

Wang, Hongzhong,et al."The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition".CELLULAR SIGNALLING 54(2019):170-178.