Recombinant DHX33 Protein Possesses Dual DNA/RNA Helicase Activity

Wang, Xingshun1,2; Ge, Wei2; Zhang, Yandong1

2019-01-29

10.1021/acs.biochem.8b00166

BIOCHEMISTRY   影响因子和分区

0006-2960

RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell proliferation. To better understand the molecular mechanism for DHX33 in exerting its biological functions, we purified recombinant DHX33 and performed biochemical studies in vitro. DHX33 protein was found to have ATPase activity that is dependent on DNA or RNA duplexes. The ATPase activity of DHX33 is coupled with its RNA/DNA unwinding activity. If a key residue in the ATP binding site were mutated, the mutant DHX33 could not unwind DNA/RNA duplexes. Furthermore, a deletion mutant of a RKK motif previously identified to be involved in ribosome DNA binding could still unwind DNA duplexes, albeit with reduced efficiency. In summary, our study reveals that purified DHX33 protein possesses unwinding activity toward DNA and RNA duplexes.

SCI ; EI

英语

第一 ; 通讯

Department of Science and Technology in Guangdong[2017A020211024]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:000457503600006

AMER CHEMICAL SOC

20182405305520

Binding sites ; Cell proliferation ; Diseases ; DNA ; Purification ; RNA ; Transcription

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Biochemistry:801.2 ; Organic Compounds:804.1

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biol, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Macau, Fac Hlth Sci, Macau, Peoples R China

Wang, Xingshun,Ge, Wei,Zhang, Yandong. Recombinant DHX33 Protein Possesses Dual DNA/RNA Helicase Activity[J]. BIOCHEMISTRY,2019,58(4):250-258.

Wang, Xingshun,Ge, Wei,&Zhang, Yandong.(2019).Recombinant DHX33 Protein Possesses Dual DNA/RNA Helicase Activity.BIOCHEMISTRY,58(4),250-258.

Wang, Xingshun,et al."Recombinant DHX33 Protein Possesses Dual DNA/RNA Helicase Activity".BIOCHEMISTRY 58.4(2019):250-258.