| 题名 | Homer Tetramer Promotes Actin Bundling Activity of Drebrin |
| 作者 | |
| 通讯作者 | Liu, Wei; Zhang, Mingjie |
| 发表日期 | 2019-01-02
|
| DOI | |
| 发表期刊 | |
| ISSN | 0969-2126
|
| EISSN | 1878-4186
|
| 卷号 | 27期号:1页码:27-+ |
| 摘要 | Drebrin is an actin bundling protein that plays critical roles in synaptic spine development and plasticity. Homer, one of the most abundant scaffolding proteins in postsynaptic density, interacts with Drebrin's C-terminal PPXXF motifs using its Ena/VASP homology 1 (EVH1) domain. However, the molecular mechanism and biological function of this interaction remain unclear. Here we show that Homer specifically binds to the first but not the second PPXXF motif in Drebrin. The crystal structure of DrebrinHomer binding motif 1 in complex with Homer EVH1 reveals a consensus Homer EVH1 binding motif. Homer tetramer promotes actin bundling activity of Drebrin in vitro and stimulates Drebrin-induced filopodia formation and elongation in cells. We further show that monomeric Homer1a antagonizes Homer1b in promoting Drebrin-stimulated actin bundling. Our study suggests a potential regulatory role of Homerl in modulating excitatory synaptic spine homeostatic scaling via binding to Drebrin. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
|
| 学校署名 | 其他
|
| 资助项目 | Shenzhen Basic Research Grants[JCYJ20160427185712266]
; Shenzhen Basic Research Grants[JCYJ20170411090807530]
|
| WOS研究方向 | Biochemistry & Molecular Biology
; Biophysics
; Cell Biology
|
| WOS类目 | Biochemistry & Molecular Biology
; Biophysics
; Cell Biology
|
| WOS记录号 | WOS:000454804900004
|
| 出版者 | |
| ESI学科分类 | BIOLOGY & BIOCHEMISTRY
|
| 来源库 | Web of Science
|
| 引用统计 |
被引频次[WOS]：18
|
| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/26639 |
| 专题 | 生命科学学院_生物系 |
| 作者单位 | 1.Shenzhen Peking Univ Hong Kong Univ Sci & Technol, Biomed Res Inst, Shenzhen Key Lab Neuronal Struct Biol, Shenzhen 518036, Peoples R China 2.Hong Kong Univ Sci & Technol, State Key Lab Mol Neurosci, Div Life Sci, Kowloon, Clear Water Bay, Hong Kong, Peoples R China 3.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Li, Zhiwei,Liu, Haiyang,Li, Jianchao,et al. Homer Tetramer Promotes Actin Bundling Activity of Drebrin[J]. STRUCTURE,2019,27(1):27-+.
|
| APA |
Li, Zhiwei.,Liu, Haiyang.,Li, Jianchao.,Yang, Qingqing.,Feng, Zhe.,...&Zhang, Mingjie.(2019).Homer Tetramer Promotes Actin Bundling Activity of Drebrin.STRUCTURE,27(1),27-+.
|
| MLA |
Li, Zhiwei,et al."Homer Tetramer Promotes Actin Bundling Activity of Drebrin".STRUCTURE 27.1(2019):27-+.
|
| 条目包含的文件 | 条目无相关文件。 | |||||
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