A New Quinone Based Fluorescent Probe for High Sensitive and Selective Detection of Biothiols and Its Application in Living Cell Imaging

Liu, Yanju1; Li, Manman1; Wong, Keith Man-Chung2; Tong, Yan1; Yang, Huaixia1; Kong, Jinming3

2019

10.1155/2019/7536431

International Journal of Analytical Chemistry   影响因子和分区

1687-8760

1687-8779

In view of the vital role of biothiols in many physiological processes, the development of simple and efficient probe for the detection of biothiols is of great medical significance. In this work, we demonstrate the use of 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which respond rapidly to biothiols especially to glutathione, as a new fluorescent probe for the selective detection and bioimaging of biothiols. This new fluorescent probe can distinguish glutathione from cysteine and homocysteine easily under physiological concentration and detect glutathione quickly within three minutes. This probe exhibits high selectivity to biothiols and the detection limit was determined to be 3.08 x 10(-9)M for glutathione, 8.55 x 10(-8)M for cysteine, and 2.17 x 10(-9)M for homocysteine, respectively. The sensing mechanism was further explored by density functional theory (DFT) and nuclear magnetic resonance (NMR) experiment; results showed that the interaction forces between the probe and biothiols were electrostatic interaction. In addition, the probe has been successfully applied to the detection of biothiols in Eca9706 cells by fluorescence confocal imaging technology.

SCI ; EI

英语

其他

Henan University of Chinese Medicine of Graduate Student Innovation Training Base Project[2017YCX037]

Chemistry

Chemistry, Analytical

WOS:000465271600001

HINDAWI LTD

20214311070060

Amino acids ; Density functional theory ; Fluorescence imaging ; Fluorescence spectroscopy ; Imaging techniques ; Nuclear magnetic resonance ; Peptides ; Physiology ; Quinone

Biology:461.9 ; Optical Devices and Systems:741.3 ; Imaging Techniques:746 ; Organic Compounds:804.1 ; Probability Theory:922.1

Web of Science

1.Henan Univ Chinese Med, Pharm Coll, Zhengzhou 450008, Henan, Peoples R China
2.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
3.Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China

Liu, Yanju,Li, Manman,Wong, Keith Man-Chung,et al. A New Quinone Based Fluorescent Probe for High Sensitive and Selective Detection of Biothiols and Its Application in Living Cell Imaging[J]. International Journal of Analytical Chemistry,2019,2019.

Liu, Yanju,Li, Manman,Wong, Keith Man-Chung,Tong, Yan,Yang, Huaixia,&Kong, Jinming.(2019).A New Quinone Based Fluorescent Probe for High Sensitive and Selective Detection of Biothiols and Its Application in Living Cell Imaging.International Journal of Analytical Chemistry,2019.

Liu, Yanju,et al."A New Quinone Based Fluorescent Probe for High Sensitive and Selective Detection of Biothiols and Its Application in Living Cell Imaging".International Journal of Analytical Chemistry 2019(2019).