南方科技大学知识苑(SUSTech KC): Naturally Occurring Single Mutations in Ebola Virus Observably Impact Infectivity

题名	Naturally Occurring Single Mutations in Ebola Virus Observably Impact Infectivity
作者	Wong, Gary 1,2,3,4; He, Shihua 1; Leung, Anders 1; Cao, Wenguang 1,3; Bi, Yuhai 2,4; Zhang, Zirui 1,3; Zhu, Wenjun 1,3; Wang, Liang 4; Zhao, Yuhui 4; Cheng, Keding 5; Liu, Di 4; Liu, Wenjun 4; Kobasa, Darwyn 1,3; Gao, George F.2,4; Qiu, Xiangguo 1,3
通讯作者	Gao, George F.; Qiu, Xiangguo
发表日期	2019-01
DOI	10.1128/JVI.01098-18
发表期刊	JOURNAL OF VIROLOGY 影响因子和分区
ISSN	0022-538X
EISSN	1098-5514
卷号	93 期号:1
摘要	Sequencing of Ebola virus (EBOV) genomes during the 2014-2016 epidemic identified several naturally occurring, dominant mutations potentially impacting virulence or tropism. In this study, we characterized EBOV variants carrying one of the following substitutions: A82V in the glycoprotein (GP), R111C in the nucleoprotein (NP), or D759G in the RNA-dependent RNA polymerase (L). Compared with the wild-type (WT) EBOV C07 isolate, NP and L mutants conferred a replication advantage in monkey Vero E6, human A549, and insectivorous bat Tb1.Lu cells, while L mutants displayed a disadvantage in human Huh7 cells. The replication of the GP mutant was significantly delayed in Tb1.Lu cells and similar to that of the WT in other cells. The L mutant was less virulent, as evidenced by increased survival for mice and a significantly delayed time to death for ferrets, but increased lengths of the period of EBOV shedding may have contributed to the prolonged epidemic. Our results show that single substitutions can have observable impacts on EBOV pathogenicity and provide a framework for the study of other mutations.
关键词	Ebola virus ferrets mice mutations pathogenicity
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	Youth Innovation Promotion Association of the Chinese Academy of Sciences (CAS)[2017122]
WOS研究方向	Virology
WOS类目	Virology
WOS记录号	WOS:000454918700004
出版者	AMER SOC MICROBIOLOGY
ESI学科分类	MICROBIOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：23
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/26729
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Publ Hlth Agcy Canada, Special Pathogens Program, Winnipeg, MB, Canada 2.Southern Univ Sci & Technol, Shenzhen Key Lab Pathogen & Immun, Guangdong Key Lab Diag & Treatment Emerging Infec, Shenzhen Peoples Hosp 3,Hosp 2, Shenzhen 518057, Peoples R China 3.Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada 4.Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China 5.Publ Hlth Agcy Canada, Natl Microbiol Lab, Monoclonal Antibody Unit, Winnipeg, MB, Canada
第一作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Wong, Gary,He, Shihua,Leung, Anders,et al. Naturally Occurring Single Mutations in Ebola Virus Observably Impact Infectivity[J]. JOURNAL OF VIROLOGY,2019,93(1).
APA	Wong, Gary.,He, Shihua.,Leung, Anders.,Cao, Wenguang.,Bi, Yuhai.,...&Qiu, Xiangguo.(2019).Naturally Occurring Single Mutations in Ebola Virus Observably Impact Infectivity.JOURNAL OF VIROLOGY,93(1).
MLA	Wong, Gary,et al."Naturally Occurring Single Mutations in Ebola Virus Observably Impact Infectivity".JOURNAL OF VIROLOGY 93.1(2019).