Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers

Zhao, Qian1,2; Pan, Tingting2,3; Xiang, Gang4; Mei, Zhipeng2; Jiang, Jiapei2; Li, Gang2; Zou, Xianshao2; Chen, Meiwan3; Sun, Dazhi2; Jiang, Shimei1; Tian, Yanqing2

2018-11-10

10.1016/j.snb.2018.06.026

SENSORS AND ACTUATORS B-CHEMICAL   影响因子和分区

0925-4005

An amphiphilic graft copolymer (P3) composed of poly(oligo (ethylene glycol) methacrylate) (POEGMA) as a hydrophilic segment and a polystyrene (PS) as a hydrophobic chain was prepared. P3 was used to incorporate a highly efficient hydrophobic oxygen probe platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) in its micelles to enable the application of PtTFPP in aqueous solution for biosensing. For achieving ratiometric oxygen sensing, a hydrophobic conjugated polymer (CP) was used as a fluorescence resonance energy transfer (FRET) donor for PtTFPP-the FRET acceptor. Results showed that diameters of these nano-oxygen sensors ranging from 50 to 60 nm and FRET efficiency as high as 98% could be achieved. Further, FRET contributed to the brightness of PtTFPP in micelles. High quantum yields of PtTFPP in non-FRET micelles and FRET micelles under nitrogen were achieved to be 0.109 and 0.231, respectively. The sensors were found to be able to measure oxygen concentrations from 0.082 to 40.9 mg/L at room temperature with linear Stern-Volmer constants. These sensors were also demonstrated to be suitable for monitoring extracellular oxygen consumption of E. Coll and HeLa cells. This study illustrated a feasible approach to achieve highly efficient ratiometric oxygen sensors without specific structure modification of the efficient PtTFPP probe, however using an amphiphilic graft copolymer approach.

Amphiphilic graft copolymer Conjugated polymer Oxygen sensor FRET Micelles Oxygen consumption

SCI ; EI

英语

通讯

Peacock Team Project from Shenzhen Science and Technology Innovation Committee[KQTD2015033110182370]

Chemistry ; Electrochemistry ; Instruments & Instrumentation

Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation

WOS:000441519000032

ELSEVIER SCIENCE SA

20182505342202

Energy transfer ; Escherichia coli ; Ethylene ; Ethylene glycol ; Forster resonance energy transfer ; Graft copolymers ; Grafting (chemical) ; Hydrophobicity ; Lanthanum compounds ; Micelles ; Oxygen sensors ; Platinum compounds ; Probes ; Solutions

Colloid Chemistry:801.3 ; Chemical Reactions:802.2 ; Organic Compounds:804.1 ; Polymeric Materials:815.1 ; Organic Polymers:815.1.1 ; Physical Properties of Gases, Liquids and Solids:931.2

CHEMISTRY

Web of Science

1.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, 2699 Qianjin Ave, Changchun 130012, Jilin, Peoples R China
2.Southern Univ Sci & Technol, Dept Mat Sci & Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
3.Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Ave Univ, Taipa, Macau, Peoples R China
4.Guangxi Normal Univ Nationalities, Sch Chem & Chem Engn, Chongzuo 532200, Guangxi, Peoples R China

Zhao, Qian,Pan, Tingting,Xiang, Gang,et al. Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,273:242-252.

Zhao, Qian.,Pan, Tingting.,Xiang, Gang.,Mei, Zhipeng.,Jiang, Jiapei.,...&Tian, Yanqing.(2018).Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers.SENSORS AND ACTUATORS B-CHEMICAL,273,242-252.

Zhao, Qian,et al."Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers".SENSORS AND ACTUATORS B-CHEMICAL 273(2018):242-252.