TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma

Xu, Hongjuan1,2,3; Yan, Xuejun1,2,3; Zhu, Hecheng4; Kang, Yuanbo1,2,3; Luo, Weiren5; Zhao, Jin1,2,3; Zhou, Kefan1,2,3; Liu, Xiwu1,2,3; Ye, Li1,2,3; Zhou, Quanwei6; Li, Shasha1,2,3; Zhao, Ming4; Wang, Lei1,2,3; Zhu, Bin1,2,3; Liu, Weidong1,2,3; Li, Jianxiong7; Jiang, Xingjun6; Ren, Caiping1,2,3

2022

10.7150/ijbs.68091

International Journal of Biological Sciences   影响因子和分区

1449-2288

Metastasis is the main cause of death in patients with nasopharyngeal carcinoma (NPC). The molecular mechanisms underlying the metastasis of NPC remain to be elucidated. TBL1X has been shown abnormally expressed in diverse cancers. However, the role and mechanism of TBL1X in NPC remain unknown. Here, we showed TBL1X expression was significantly higher in metastatic NPC tissues compared to non-metastatic tissues and significantly correlated with TNM stage and metastasis of NPC patients. In addition, NPC patients with high TBL1X expression had a poor prognosis. TBL1X interacted with TCF4 to trans-activate Flot2 expression. TBL1X promoted NPC cell migration and invasion in vitro and in vivo through Flot2. Moreover, Flot2 increased the expression of TBL1X by upregulating c-myc, which was identified to be a positively regulatory transcription factor of TBL1X. TBL1X could restore the functional changes of NPC cells resulting from Flot2 alteration. TBL1X and Flot2 were positively correlated in NPC. Patients with high expression of both TBL1X and Flot2 possessed poorer overall survival (OS) and disease-free survival (DFS) compared to patients with high expression of any single one of the two proteins. Our findings demonstrate that TBL1X and Flot2 positively regulate each other to promote NPC metastasis, which provides novel potential molecular targets for NPC treatment.

Nasopharyngeal carcinoma (NPC) metastasis prognosis Transducin beta-like protein 1 X (TBL1X) Flotillin-2 (Flot2)

SCI

英语

其他

National Natural Science Foundation of China[81773179,81272972,81872202] ; Natural Science Foundation of Hunan Province[2020JJ4771] ; Hunan Provincial Science and Technology Project[2014FJ6006] ; Natural Science Foundation of Guangdong Province[2018A030313778] ; Independent Exploration and Innovation Project of Central South University[2021zzts0929]

Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics

Biochemistry & Molecular Biology ; Biology

WOS:000743245200004

IVYSPRING INT PUBL

Web of Science

1.Cent South Univ, Natl Clin Res Ctr Geriatr Disorders, Canc Res Inst, Dept Neurosurg,NHC Key Lab Biol Nanotechnol,Xiang, Changsha 410008, Hunan, Peoples R China
2.Cent South Univ, Sch Basic Med Sci, Chinese Minist Educ, NHC Key Lab Carcinogenesis, Changsha 410008, Hunan, Peoples R China
3.Cent South Univ, Sch Basic Med Sci, Chinese Minist Educ, Key Lab Carcinogenesis & Canc Invas, Changsha 410008, Hunan, Peoples R China
4.Changsha Kexin Canc Hosp, Changsha 410008, Hunan, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Canc Res Inst, Shenzhen 518112, Guangdong, Peoples R China
6.Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Dept Neurosurg, Changsha 410008, Hunan, Peoples R China
7.Chinese Peoples Liberat Army Gen Hosp, Dept Radiotherapy, Beijing 100089, Peoples R China

Xu, Hongjuan,Yan, Xuejun,Zhu, Hecheng,et al. TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma[J]. International Journal of Biological Sciences,2022,18(3).

Xu, Hongjuan.,Yan, Xuejun.,Zhu, Hecheng.,Kang, Yuanbo.,Luo, Weiren.,...&Ren, Caiping.(2022).TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.International Journal of Biological Sciences,18(3).

Xu, Hongjuan,et al."TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma".International Journal of Biological Sciences 18.3(2022).