One-pot synthesized organosilica nanospheres for multiplexed fluorescent nanobarcoding and subcellular tracking

Du, Xinfeng1; Wang, Yifu1; Zhai, Jingying2; Guo, Chao1; Zhang, Yupu1; Huang, Wenyu; Ma, Xueqing2; Xie, Xiaojiang1

2021-12-01

10.1039/d1nr06540h

Nanoscale   影响因子和分区

2040-3364

2040-3372

Multicolor microbeads are widely used in flow cytometry for various cellular and immunoassays. However, they are limited by their large size of around one to tens of micrometers. Nanomaterials for multiplexed analysis are emerging as valuable tools in high-throughput assays and fluorescence cell barcoding. We present barcoding and related cellular studies based on mass-produced organosilane-derived multifunctional nanospheres with a uniform size. Functional groups including thiols, amines, and azides were integrated in one step from various organosilanes without additional orthosilicates. Fluorescent nanobarcodes (NBs) were achieved through flexible physical adsorption and chemical ligation of spectrally separated fluorescent dyes. Live cells labeled with the NBs were readily distinguished by flow cytometry. The NBs have a small and uniform size (ca. 27 nm in diameter), excellent biocompatibility, rapid cellular uptake, and low dye leakage. The fluorescent nanospheres were applied for long-term cell tracking during multiple rounds of cell division and monitored over 48 hours. While most nanospheres were endolysosome-targeting, modification with fluorescein isothiocyanate (FITC) surprisingly lighted up the cell nucleus. This work lays the foundation of a unique family of functional nanomaterials promising for multiplex detection and other chemical and biological applications.

SCI ; EI

英语

第一 ; 通讯

Shenzhen Municipal Science and Technology Innovation Council[JCYJ20180504165819965] ; National Natural Science Foundation of China[B040401]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000742410300001

ROYAL SOC CHEMISTRY

20220711647353

Amines ; Biocompatibility ; Cell proliferation ; Chemical detection ; Flow cytometry ; Fluorescence ; Nanostructured materials ; Silica

Biology:461.9 ; Immunology:461.9.1 ; Light/Optics:741.1 ; Optical Devices and Systems:741.3 ; Nanotechnology:761 ; Chemistry:801 ; Organic Compounds:804.1 ; Solid State Physics:933 ; Crystalline Solids:933.1 ; Optical Variables Measurements:941.4

Web of Science

1.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China

Du, Xinfeng,Wang, Yifu,Zhai, Jingying,et al. One-pot synthesized organosilica nanospheres for multiplexed fluorescent nanobarcoding and subcellular tracking[J]. Nanoscale,2021,14:1787-1795.

Du, Xinfeng.,Wang, Yifu.,Zhai, Jingying.,Guo, Chao.,Zhang, Yupu.,...&Xie, Xiaojiang.(2021).One-pot synthesized organosilica nanospheres for multiplexed fluorescent nanobarcoding and subcellular tracking.Nanoscale,14,1787-1795.

Du, Xinfeng,et al."One-pot synthesized organosilica nanospheres for multiplexed fluorescent nanobarcoding and subcellular tracking".Nanoscale 14(2021):1787-1795.