Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-kappa B signaling

Tong, Haibo1,2; Zou, Chunlin1; Qin, Siyuan1,2; Meng, Jie1,2; Keller, Evan T.3; Zhang, Jian2,3; Lu, Yi1,2

2018-09

10.7555/JBR.32.20180035

Journal of Biomedical Research   影响因子和分区

1674-8301

1876-4819

Prostate cancer preferentially metastasizes to the bone. However, the underlying molecular mechanisms are still unclear. To explore the effects of a bone-mimicking microenvironment on PC3 prostate cancer cell growth and metastasis, we used osteoblast differentiation medium (ODM; minimal essential medium alpha supplemented with L-ascorbic acid) to mimic the bone microenvironment. PC3 cells grown in ODM underwent epithelial-mesenchymal transition and showed enhanced colony formation, migration, and invasion abilities compared to the cells grown in normal medium. PC3 cells grown in ODM showed enhanced metastasis when injected in mice. A screening of signaling pathways related to invasion and metastasis revealed that the NF-kappa B pathway was activated, which could be reversed by Bay 11-7082, a NF-kappa B pathway inhibitor. These results indicate that the cells in different culture conditions manifested significantly different biological behaviors and the NF-kappa B pathway is a potential therapeutic target for prostate cancer bone metastasis.

prostate cancer metastasis NF-kappa B Bay 11-7082 EMT

ESCI

英语

其他

National Natural Science Foundation of China (NSFC)[81272415][81171993] ; NSFC Key Project[81130046] ; Guangxi Key Projects[2013-GXNSFEA053004] ; Guangxi Projects[2014GXNS-FDA118030]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000451872600004

NANJING MEDICAL UNIV

Web of Science

1.Guangxi Med Univ, Key Lab Longev & Aging Related Dis, Minist Educ, Nanning 530021, Guangxi, Peoples R China;
2.Southern Univ Sci & Technol, Sch Med, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China;
3.Univ Michigan, Dept Pathol & Internal Med, Ann Arbor, MI 48109 USA

Tong, Haibo,Zou, Chunlin,Qin, Siyuan,et al. Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-kappa B signaling[J]. Journal of Biomedical Research,2018,32(5):343-353.

Tong, Haibo.,Zou, Chunlin.,Qin, Siyuan.,Meng, Jie.,Keller, Evan T..,...&Lu, Yi.(2018).Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-kappa B signaling.Journal of Biomedical Research,32(5),343-353.

Tong, Haibo,et al."Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-kappa B signaling".Journal of Biomedical Research 32.5(2018):343-353.