Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction

Dou, Yuhong1; Li, Yuxia1; Ma, Caifeng1; Zhu, Huijun1; Du, Jikun1; Liu, Helu1; Liu, Qiong1; Chen, Rui2; Tan, Ying3

2018-09

10.3892/mmr.2018.9253

Molecular Medicine Reports   影响因子和分区

1791-2997

1791-3004

Human adenovirus (HAdV) is increasingly recognized as a major cause of human respiratory tract viral infections. Its outbreaks and epidemics in various populations resulted in considerable morbidity and mortality. Therefore, a rapid and specific assay for HAdV in clinical samples is of crucial importance to diagnosing HAdV infections. The present study aimed to develop and evaluate a multiplex quantitative polymerase chain reaction (qPCR) assay for the rapid detection and accurate quantification of HAdV B, C and E. The lower limit of detection for this assay was two genomic copies per reaction, and quantitative linearity ranged from 2 to 2x10 6 copies per reaction of the input viral DNA. Furthermore, 3,160 throat swab samples that tested HAdV negative by the immunofluorescence assay were collected and retested using the multiplex qPCR assay. The results showed that 2,906 samples were HAdV negative and the other 254 samples were HAdV positive. The HAdV species identified included B (184 samples), C (51 samples), and E (39 samples). Among the three HAdV species, HAdV B and F were detected from 8 samples, and HAdV C and F were detected from other 12 samples. The overall results demonstrated that the sensitivity and specificity of the proposed assay were 100% (254/254) and 99.6% (2894/2906), respectively. From the perspective of routine clinical diagnosis, this assay represented a rapid (s1.5 h) and economic strategy, and had the potential to be used for the rapid and accurate diagnosis of human respiratory infections caused by HAdV B, C and F.

human adenovirus multiplex qPCR respiratory tract throat swab samples

SCI

英语

其他

Shenzhen Bao'an Science and Technology Project[2015204]

Oncology ; Research & Experimental Medicine

Oncology ; Medicine, Research & Experimental

WOS:000442890400041

SPANDIDOS PUBL LTD

Web of Science

1.Guangzhou Med Univ, Shenzhen Shajing Hosp, Dept Clin Lab, 3 Shajing St, Shenzhen 518104, Guangdong, Peoples R China
2.Second Peoples Hosp Futian Dist, Dept Clin Lab, 27 Zhongkang Rd, Shenzhen 518049, Guangdong, Peoples R China
3.South Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China

Dou, Yuhong,Li, Yuxia,Ma, Caifeng,et al. Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction[J]. Molecular Medicine Reports,2018,18(3):2889-2897.

Dou, Yuhong.,Li, Yuxia.,Ma, Caifeng.,Zhu, Huijun.,Du, Jikun.,...&Tan, Ying.(2018).Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction.Molecular Medicine Reports,18(3),2889-2897.

Dou, Yuhong,et al."Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction".Molecular Medicine Reports 18.3(2018):2889-2897.