In vivo study of rat cortical hemodynamics using a stereotaxic-apparatus-compatible photoacoustic microscope

Guo, Heng1,2; Chen, Qian1,2; Qi, Weizhi1,2; Chen, Xingxing2; Xi, Lei1,2

2018-09

10.1002/jbio.201800067

Journal of Biophotonics   影响因子和分区

1864-063X

1864-0648

Brain imaging is an important technique in cognitive neuroscience. In this article, we designed a stereotaxic-apparatus-compatible photoacoustic microscope for the studies of rat cortical hemodynamics. Compared with existing optical resolution photoacoustic microscopy (ORPAM) systems, the probe owns feature of fast, light and miniature. In this microscope, we integrated a miniaturized ultrasound transducer with a center frequency of 10 MHz to detect photoacoustic signals and a 2-dimensional (2D) microelectromechanical system (MEMS) scanner to achieve raster scanning of the optical focus. Based on phantom evaluation, this imaging probe has a high lateral resolution of 3.8 mu m and an effective imaging domain of 2 x 2 mm(2). Different from conventional ORPAMs, combining with standard stereotaxic apparatus enables broad studies of rodent brains without any motion artifact. To show its capability, we successfully captured red blood cell flow in the capillary, monitored the vascular changes during bleeding and blood infusion and visualized cortical hemodynamics induced by middle cerebral artery occlusion.

brain imaging cortical hemodynamics photoacoustic microscope

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China[61775028] ; National Natural Science Foundation of China[81571722] ; National Natural Science Foundation of China[61528401]

Biochemistry & Molecular Biology ; Biophysics ; Optics

Biochemical Research Methods ; Biophysics ; Optics

WOS:000443944500020

WILEY-V C H VERLAG GMBH

20182005189204

Acoustic microscopes ; Blood ; Brain ; Brain mapping ; Electromechanical devices ; Hemodynamics ; MEMS ; Microscopes ; Probes ; Rats ; Ultrasonic applications ; Ultrasonic transducers

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Electric Equipment:704.2 ; Optical Devices and Systems:741.3 ; Imaging Techniques:746 ; Acoustic Devices:752.1 ; Ultrasonic Devices:753.2 ; Ultrasonic Applications:753.3

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Elect Sci & Technol China, Sch Phys, Chengdu, Sichuan, Peoples R China

Guo, Heng,Chen, Qian,Qi, Weizhi,et al. In vivo study of rat cortical hemodynamics using a stereotaxic-apparatus-compatible photoacoustic microscope[J]. Journal of Biophotonics,2018,11(9).

Guo, Heng,Chen, Qian,Qi, Weizhi,Chen, Xingxing,&Xi, Lei.(2018).In vivo study of rat cortical hemodynamics using a stereotaxic-apparatus-compatible photoacoustic microscope.Journal of Biophotonics,11(9).

Guo, Heng,et al."In vivo study of rat cortical hemodynamics using a stereotaxic-apparatus-compatible photoacoustic microscope".Journal of Biophotonics 11.9(2018).