Stimulation of the Production of Prostaglandin E-2 by Ethyl Gallate, a Natural Phenolic Compound Richly Contained in Longan

Wang, Hui Rong1; Sui, Hao Chen2; Ding, Yan Yan1; Zhu, Bao Ting1,2

2018-09

10.3390/biom8030091

Biomolecules   影响因子和分区

2218-273X

Ethyl gallate is a phenolic compound richly contained in Longan. In traditional Chinese medicine, Longan is widely known as a fruit with hot properties, with a tendency to promote inflammatory and certain other responses. The mechanism for its proinflammatory as well as health beneficial effects is poorly understood. Based on our earlier observation that certain natural phenolic compounds can serve as reducing cosubstrates for cyclooxygenases (COXs), we sought to test a hypothesis that ethyl gallate may activate the catalytic activity of the COX enzymes. Results from studies using cultured cells and animals show that ethyl gallate can activate the production of prostaglandin E-2, a representative prostaglandin tested in this study. Computational analysis indicates that ethyl gallate can activate the peroxidase active sites of COX-1 and COX-2 by serving as a reducing cosubstrate. The effect of ethyl gallate is abrogated by galangin, which is known to bind to the same peroxidase active sites of COX-1 and COX-2 as a competitive inhibitor. The findings of this study offer support for a novel hypothesis that the proinflammatory as well as health beneficial effects of Longan may be partly attributable to the activation of COX-1 and COX-2 by ethyl gallate.

ethyl gallate cyclooxygenase peroxidase activity mechanism of catalytic activation

SCI

英语

第一 ; 通讯

Shenzhen Peacock Plan[KQTD2016053117035204]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:000448394900049

MDPI

Web of Science

1.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China
2.Chinese Univ Hong Kong, Sch Sci & Engn, Shenzhen 518172, Peoples R China

Wang, Hui Rong,Sui, Hao Chen,Ding, Yan Yan,et al. Stimulation of the Production of Prostaglandin E-2 by Ethyl Gallate, a Natural Phenolic Compound Richly Contained in Longan[J]. Biomolecules,2018,8(3).

Wang, Hui Rong,Sui, Hao Chen,Ding, Yan Yan,&Zhu, Bao Ting.(2018).Stimulation of the Production of Prostaglandin E-2 by Ethyl Gallate, a Natural Phenolic Compound Richly Contained in Longan.Biomolecules,8(3).

Wang, Hui Rong,et al."Stimulation of the Production of Prostaglandin E-2 by Ethyl Gallate, a Natural Phenolic Compound Richly Contained in Longan".Biomolecules 8.3(2018).