南方科技大学知识苑(SUSTech KC): Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance

题名	Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance
作者	Yang, Yang 1,2 ; Li, Shanqin 1,2; Wong, Gary 1,2; Ma, Sufang 2; Xu, Zhixiang 1; Zhao, Xiaonan 3; Li, Hong 3; Xu, Wen 3; Zheng, Haixia1 ; Lin, Jingyan 1; Zhao, Qi1 ; Liu, Wenjun 2; Liu, Yingxia 1,5; Gao, George F.1,2,4,5; Bi, Yuhai 1,2
通讯作者	Bi, Yuhai
发表日期	2018-08-15
DOI	10.1186/s12879-018-3302-7
发表期刊	BMC INFECTIOUS DISEASES 影响因子和分区
ISSN	1471-2334
卷号	18
摘要	Background: During the fifth wave of human H7N9 infections, a novel highly pathogenic (HP) H7N9 variant emerged with an insertion of multiple basic amino acids in the HA cleavage site. Moreover, a neuraminidase inhibitor (NAI) resistance (R292K in NA) mutation was found in H7N9 isolates from humans, poultry and the environment. In this study, we set out to develop and validate a multiplex quantitative reverse transcript polymerase chain reaction (qRT-PCR) to simultaneously detect the presence of H7N9 and further identify the HP and NAI-resistance mutations.
关键词	Highly/low Pathogenic Avian Influenza Virus H7n9 Quadruple Qrt-pcr Molecular Diagnostics Nai-resistance Oseltamivir
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Youth Innovation Promotion Association of Chinese Academy of Sciences[2017122]
WOS研究方向	Infectious Diseases
WOS类目	Infectious Diseases
WOS记录号	WOS:000442090300004
出版者	BMC
ESI学科分类	IMMUNOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：12
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/27373
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Hosp 2, State Key Discipline Infect Dis,Shenzhen Key Lab, Shenzhen 518112, Peoples R China 2.Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, Inst Microbiol,CAS Key Lab Pathogen Microbiol & I, Collaborat Innovat Ctr Diag & Treatment Infect Di, Beijing 100101, Peoples R China 3.Yunnan Ctr Dis Control & Prevent Kunming, Kunming 650022, Yunnan, Peoples R China 4.Chinese Ctr Dis Control & Prevent China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China 5.Univ Chinese Acad Sci, Med Sch, Chinese Acad Sci, Beijing 101408, Peoples R China
第一作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Yang, Yang,Li, Shanqin,Wong, Gary,et al. Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance[J]. BMC INFECTIOUS DISEASES,2018,18.
APA	Yang, Yang.,Li, Shanqin.,Wong, Gary.,Ma, Sufang.,Xu, Zhixiang.,...&Bi, Yuhai.(2018).Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance.BMC INFECTIOUS DISEASES,18.
MLA	Yang, Yang,et al."Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance".BMC INFECTIOUS DISEASES 18(2018).

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