南方科技大学知识苑(SUSTech KC): Ratiometric 4Pi single-molecule localization with optimal resolution and color assignment

题名	Ratiometric 4Pi single-molecule localization with optimal resolution and color assignment
作者	Chen, Jianwei 1,2 ; Yao, Benxi 1; Yang, Zhichao 1,3 ; Shi, Wei1 ; Luo, Tingdan1 ; Xi, Peng 1,4; Jin, Dayong1,3 ; Li, Yiming1
通讯作者	Li, Yiming
发表日期	2022-01-15
DOI	10.1364/OL.446987
发表期刊	OPTICS LETTERS 影响因子和分区
ISSN	0146-9592
EISSN	1539-4794
卷号	47 期号:2页码:325-328
摘要	4Pi single-molecule localization microscopy (4Pi-SMLM) with two opposing objectives achieves sub-10 nm isotropic 3D resolution when as few as 250 photons are collected by each objective. Here, we develop a new ratiometric multicolor imaging strategy for 4Pi-SMLM that employs the intrinsic multi-phase interference intensity without increasing the complexity of the system and achieves both optimal 3D resolution and color separation. By partially linking the photon parameters between channels with an interference difference of p during global fitting of the multi-channel 4Pi single-molecule data, we show via simulated data that the loss of localization precision is minimal compared with the theoretical minimum uncertainty, the Cramer-Rao lower bound. (C) 2022 Optica Publishing Group
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	第一 ; 通讯
资助项目	Natural Science Foundation of Guangdong Province[2020A1515110380] ; Shenzhen Municipal Science and Technology Innovation Council["KQTD20200820113012029","KQTD20170810110913065",20200925174735005] ; Key Technology Research and Development Program of Shandong[2021CXGC010212]
WOS研究方向	Optics
WOS类目	Optics
WOS记录号	WOS:000743361600031
出版者	OPTICAL SOC AMER
EI入藏号	20220311469172
EI主题词	Molecules
EI分类号	Atomic and Molecular Physics:931.3
ESI学科分类	PHYSICS
来源库	Web of Science
引用统计	被引频次[WOS]：5
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/273775
专题	工学院_生物医学工程系
作者单位	1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China 2.Harbin Inst Technol, Harbin 150001, Peoples R China 3.Univ Technol Sydney, Fac Sci, Inst Biomed Mat & Devices IBMD, Sydney, NSW 2007, Australia 4.Peking Univ, Coll Future Technol, Dept Biomed Engn, Beijing 100871, Peoples R China
第一作者单位	生物医学工程系
通讯作者单位	生物医学工程系
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Chen, Jianwei,Yao, Benxi,Yang, Zhichao,et al. Ratiometric 4Pi single-molecule localization with optimal resolution and color assignment[J]. OPTICS LETTERS,2022,47(2):325-328.
APA	Chen, Jianwei.,Yao, Benxi.,Yang, Zhichao.,Shi, Wei.,Luo, Tingdan.,...&Li, Yiming.(2022).Ratiometric 4Pi single-molecule localization with optimal resolution and color assignment.OPTICS LETTERS,47(2),325-328.
MLA	Chen, Jianwei,et al."Ratiometric 4Pi single-molecule localization with optimal resolution and color assignment".OPTICS LETTERS 47.2(2022):325-328.