Membrane nanodomains modulate formin condensation for actin remodeling in Arabidopsis innate immune responses

Ma, Zhiming1; Sun, Yanbiao2; Zhu, Xinlu1; Yang, Liang1,3,4; Chen, Xu2; Miao, Yansong1

2022

10.1093/plcell/koab261

PLANT CELL   影响因子和分区

1040-4651

1532-298X

The assembly of macromolecules on the plasma membrane concentrates cell surface biomolecules into nanometer- to micrometer-scale clusters (nano- or microdomains) that help the cell initiate or respond to signals. In plant-microbe interactions, the actin cytoskeleton undergoes rapid remodeling during pathogen-associated molecular pattern-triggered immunity (PTI). The nanoclustering of formin-actin nucleator proteins at the cell surface has been identified as underlying actin nucleation during plant innate immune responses. Here, we show that the condensation of nanodomain constituents and the self-assembly of remorin proteins enables this mechanism of controlling formin condensation and activity during innate immunity in Arabidopsis thaliana. Through intrinsically disordered region-mediated remorin oligomerization and formin interaction, remorin gradually recruits and condenses formins upon PTI activation in lipid bilayers, consequently increasing actin nucleation in a time-dependent manner postinfection. Such nanodomain- and remorin-mediated regulation of plant surface biomolecules is expected to be a general feature of plant innate immune responses that creates spatially separated biochemical compartments and fine tunes membrane physicochemical properties for transduction of immune signals in the host.

SCI

英语

NI论文

其他

National Science Foundation of China[31870170] ; Nanyang Technological University (NTU) Singapore startup grant[M4081533] ; Singapore Ministry of Education (MOE) Tier 1[RG32/20] ; Singapore Ministry of Education (MOE) Tier 3[MOE2019-T3-1-012]

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

WOS:000745840600021

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore
2.Fujian Agr & Forestry Univ, Hort Plant Biol & Metabol Ctr, Haixia Inst Sci & Technol, Fuzhou 350002, Peoples R China
3.Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551, Singapore
4.Southern Univ Sci & Technol, Sch Med, 1088 Xueyuan Blvd, Shenzhen 518055, Peoples R China

Ma, Zhiming,Sun, Yanbiao,Zhu, Xinlu,et al. Membrane nanodomains modulate formin condensation for actin remodeling in Arabidopsis innate immune responses[J]. PLANT CELL,2022,34(1).

Ma, Zhiming,Sun, Yanbiao,Zhu, Xinlu,Yang, Liang,Chen, Xu,&Miao, Yansong.(2022).Membrane nanodomains modulate formin condensation for actin remodeling in Arabidopsis innate immune responses.PLANT CELL,34(1).

Ma, Zhiming,et al."Membrane nanodomains modulate formin condensation for actin remodeling in Arabidopsis innate immune responses".PLANT CELL 34.1(2022).