Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains

Tuan Minh Tran1,6; Chng, Choon-Peng2; Pu, Xiaoming1,7; Ma, Zhiming1; Han, Xiao1; Liu, Xiaolin1; Yang, Liang3,4; Huang, Changjin2,5; Miao, Yansong1

2022

10.1093/plcell/koab276

PLANT CELL   影响因子和分区

1040-4651

1532-298X

Outer membrane vesicles (OMVs) are released from the outer membranes of Gram-negative bacteria during infection and modulate host immunity during host-pathogen interactions. The mechanisms by which OMVs are perceived by plants and affect host immunity are unclear. Here, we used the pathogen Xanthomonas campestris pv. campestris to demonstrate that OMV-plant interactions at the Arabidopsis thaliana plasma membrane (PM) modulate various host processes, including endocytosis, innate immune responses, and suppression of pathogenesis by phytobacteria. The lipid phase of OMVs is highly ordered and OMVs directly insert into the Arabidopsis PM, thereby enhancing the plant PM's lipid order; this also resulted in strengthened plant defenses. Strikingly, the integration of OMVs into the plant PM is host nanodomain- and remorin-dependent. Using coarse-grained simulations of molecular dynamics, we demonstrated that OMV integration into the plant PM depends on the membrane lipid order. Our computational simulations further showed that the saturation level of the OMV lipids could fine-tune the enhancement of host lipid order. Our work unraveled the mechanisms underlying the ability of OMVs produced by a plant pathogen to insert into the host PM, alter host membrane properties, and modulate plant immune responses.

SCI

英语

NI论文

其他

Nanyang Technological University startup grant["M4081533","M4082352"] ; Ministry of Education (MOE) Tier 1["RG32/20","RT11/20","RG92/19"] ; Ministry of Education (MOE) Tier 3[MOE2019-T31-012] ; Accelerating Creativity and Excellence grant[NTUACE2020-07]

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

WOS:000745840600022

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore
2.Nanyang Technol Univ, Sch Mech & Aerosp Engn, Singapore 639798, Singapore
3.Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551, Singapore
4.Southern Univ Sci & Technol, Sch Med, Guangzhou, Peoples R China
5.Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637459, Singapore
6.Univ S Alabama, Dept Biol, Mobile, AL 36688 USA
7.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Guangdong, Peoples R China

Tuan Minh Tran,Chng, Choon-Peng,Pu, Xiaoming,et al. Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains[J]. PLANT CELL,2022,34(1).

Tuan Minh Tran.,Chng, Choon-Peng.,Pu, Xiaoming.,Ma, Zhiming.,Han, Xiao.,...&Miao, Yansong.(2022).Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains.PLANT CELL,34(1).

Tuan Minh Tran,et al."Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains".PLANT CELL 34.1(2022).