Development and characterization of cannabidiol-loaded alginate copper hydrogel for repairing open bone defects in vitro

Qi，Jianchao1,2,3,5; Zheng，Zhe2; Hu，Liqiu2; Wang，Huizhen2,5; Tang，Bin2,4,5; Lin，Lijun1

2022-04-01

10.1016/j.colsurfb.2022.112339

COLLOIDS AND SURFACES B-BIOINTERFACES   影响因子和分区

0927-7765

1873-4367

The clinical treatment of open bone defects caused by accidental bone trauma, bone tumors, bone diseases and bone infections is challenging. In this study, we designed and fabricated a multifunctional alginate-based hydrogel that contains cannabidiol (CBD), SA@Cu/CBD hydrogel, for repairing open bone defects. The results of physicochemical characterization showed that the SA@Cu/CBD hydrogel was successfully prepared and showed a suitable swelling ratio, high thermal stability, and stable mechanical properties. In vitro evaluation of antibacterial activity indicated that more than 90% of S. aureus and E. coli were inhibited compared to the control group. The ALP activity assay showed that the ALP expression level of MC3T3-E1cells in SA@Cu/CBD hydrogel was approximately 2-fold higher than that in the control group on day 7 and 14. Additionally, compared to the control group, the level of mineralized deposits in SA@Cu/CBD hydrogel was also improved by about 2 times on day 14. The PCR results indicated the mRNA expression levels of osteogenic markers (ALP, Col1α1, OCN, and RUNX2 genes) and angiogenic markers (EGFL6 and VEGF genes) in SA@Cu/CBD hydrogel were significantly upregulated compared to that in the control group, and the mRNA expression levels of critical inflammatory cytokines (TNF-α and IL-1β) in the SA@Cu/CBD hydrogel were significantly down-regulated compared to that in SA@Cu hydrogel. Taken together, these results demonstrated that the SA@Cu/CBD hydrogel showed significantly anti-bacterial, anti-inflammation, angiogenic and osteogenic activities in vitro studies. Thus, SA@Cu/CBD hydrogels may be a promising candidate in repairing open bone defects.

Alginate Anti-inflammation Antibacterial properties Bone defect Cannabidiol

SCI ; EI

英语

通讯

National Key Research and Development Program of China[2019YFA0906004] ; Na-tional Foundation of Science and Technology[11872200] ; Guangdong Foundation of Science and Technology[2017B030301018] ; Shenzhen Science and Technology Innovation Committee[JSGG20200225151916021]

Biophysics ; Chemistry ; Materials Science

Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials

WOS:000796512600003

ELSEVIER

20220511568692

Copper ; Defects ; Escherichia coli ; Genes ; Hydrogels ; Pathology ; Repair

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Copper:544.1 ; Colloid Chemistry:801.3 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Maintenance:913.5 ; Materials Science:951

BIOLOGY & BIOCHEMISTRY

2-s2.0-85123733951

Scopus

1.Department of Joint and Orthopedics,Zhujiang Hospital,Southern Medical University,Guangzhou,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China
3.Department of Emergency surgery,Shengli Clinical Medical College of Fujian Medical University,Fujian Provincial Hospital,Fuzhou,China
4.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,China
5.Shenzhen Key Laboratory of Cell Microenvironment,China

Qi，Jianchao,Zheng，Zhe,Hu，Liqiu,et al. Development and characterization of cannabidiol-loaded alginate copper hydrogel for repairing open bone defects in vitro[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2022,212.

Qi，Jianchao,Zheng，Zhe,Hu，Liqiu,Wang，Huizhen,Tang，Bin,&Lin，Lijun.(2022).Development and characterization of cannabidiol-loaded alginate copper hydrogel for repairing open bone defects in vitro.COLLOIDS AND SURFACES B-BIOINTERFACES,212.

Qi，Jianchao,et al."Development and characterization of cannabidiol-loaded alginate copper hydrogel for repairing open bone defects in vitro".COLLOIDS AND SURFACES B-BIOINTERFACES 212(2022).