A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices

Perumal，Madhusoothanan B.1; Sah，Pankaj1,2

2022-03-18

10.1016/j.xpro.2021.101085

STAR Protocols   影响因子和分区

2666-1667

Basolateral amygdala circuits generate oscillatory network activity to process and remember emotion-tagged events. Ex vivo preparations that recapitulate network activities seen in vivo provide an ideal system to investigate the mechanisms driving these network oscillations. Here we describe an ex vivo preparation of basolateral amygdala slices from rodents for measuring the generated sharp wave ripple oscillations (SWs) using local field potential recording and targeted recording from chandelier neurons that initiate SWs. For complete details on the use and execution of this protocol, please refer to Perumal et al. (2021).

Cell Biology Cognitive Neuroscience Model Organisms Neuroscience

英语

通讯

2-s2.0-85122618790

Scopus

1.Queensland Brain Institute,Brisbane,4072,Australia
2.Joint Center for Neuroscience and Neural Engineering and Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China

Perumal，Madhusoothanan B.,Sah，Pankaj. A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices[J]. STAR Protocols,2022,3(1).

Perumal，Madhusoothanan B.,&Sah，Pankaj.(2022).A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices.STAR Protocols,3(1).

Perumal，Madhusoothanan B.,et al."A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices".STAR Protocols 3.1(2022).