A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish

Liao, Qiwen1,2; Gong, Guiyi1,2; Siu, Shirley Weng In3; Wong, Clarence Tsun Ting4; Yu, Huidong5; Tse, Yu Chung6; Radis-Baptista, Gandhi7; Lee, Simon Ming-Yuen1,2

2018-06

10.3390/toxins10060238

Toxins   影响因子和分区

2072-6651

Palythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcriptome of P. caribaeroum was deep sequenced, and a diversity of toxin-related peptide sequences were identified, and some retrieved for functional analysis. In this work, a peptide precursor containing a ShK domain, named PcShK3, was analyzed by means of computational processing, comprising structural phylogenetic analysis, model prediction, and dynamics simulation of peptide-receptor interaction. The combined data indicated that PcShK3 is a distinct peptide which is homologous to a cluster of peptides belonging to the ShK toxin family. In vivo, PcShK3 distributed across the vitelline membrane and accumulated in the yolk sac stripe of zebrafish larvae. Notably, it displayed a significant cardio-protective effect in zebrafish in concentrations inferior to the IC50 (<43.53 +/- 6.45 mu M), while in high concentrations (>IC50), it accumulated in the blood and caused pericardial edema, being cardiotoxic to zebrafish larvae. Remarkably, PcShK3 suppressed the 6-OHDA-induced neurotoxicity on the locomotive behavior of zebrafish. The present results indicated that PcShK3 is a novel member of ShK toxin family, and has the intrinsic ability to induce neuro- and cardio-protective effects or cause cardiac toxicity, according to its effective concentration.

zoantharian ShK-like peptide dynamics simulation voltage-gated K+ ion channel calcium-activated K+ ion channel animal toxins

SCI

英语

其他

Brazilian National Council for Scientific and Technological Development, CNPq (Marine Biotechnology Network)[408835/2013-3]

Food Science & Technology ; Toxicology

Food Science & Technology ; Toxicology

WOS:000436131800026

MDPI

Web of Science

1.Univ Macau, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
3.Univ Macau, Fac Sci & Technol, Dept Comp & Informat Sci, Macau, Peoples R China
4.Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong, Peoples R China
5.Shenzhen Rongxin Biotechnol Co Ltd, Shenzhen 518054, Peoples R China
6.South Univ Sci & Technol China, Dept Biol, Shenzhen 518055, Peoples R China
7.Univ Fed Ceara, Inst Marine Sci, Lab Biochem & Biotechnol, BR-60165081 Fortaleza, Ceara, Brazil

Liao, Qiwen,Gong, Guiyi,Siu, Shirley Weng In,et al. A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish[J]. Toxins,2018,10(6).

Liao, Qiwen.,Gong, Guiyi.,Siu, Shirley Weng In.,Wong, Clarence Tsun Ting.,Yu, Huidong.,...&Lee, Simon Ming-Yuen.(2018).A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish.Toxins,10(6).

Liao, Qiwen,et al."A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish".Toxins 10.6(2018).