Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology

Xu, Ruilian1; Tang, Jun1,2,3,4; Deng, Quantong1; He, Wan1; Sun, Xiujie2; Xia, Ligang1; Cheng, Zhiqiang1; He, Lisheng1; You, Shuyuan1; Hu, Jintao1; Fu, Yuxiang1; Zhu, Jian1; Chen, Yixin1; Gao, Weina2; He, An2; Guo, Zhengyu2; Lin, Lin2; Li, Hua; Hu, Chaofeng3; Tian, Ruijun2,5

2018-05

10.1021/acs.analchem.8b00596

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Increasing attention has been focused on cell type proteome profiling for understanding the heterogeneous multicellular microenvironment in tissue samples. However, current cell type proteome profiling methods need large amounts of starting materials which preclude their application to clinical tumor specimens with limited access. Here, by seamlessly combining laser capture microdissection and integrated proteomics sample preparation technology SIS-PROT, specific cell types in tumor samples could be precisely dissected with single cell resolution and processed for high sensitivity proteome profiling. Sample loss and contamination due to the multiple transfer steps are significantly reduced by the full integration and noncontact design. H&E staining dyes which are necessary for cell type investigation could be selectively removed by the unique two-stage design of the spintip device. This easy-to-use proteome profiling technology achieved high sensitivity with the identification of more than 500 proteins from only 0.1 mm(2) and 10 mu m thickness colon cancer tissue section. The first cell type proteome profiling of four cell types from one colon tumor and surrounding normal tissue, including cancer cells, enterocytes, lymphocytes, and smooth muscle cells, was obtained. 5271, 4691, 4876, and 2140 protein groups were identified, respectively, from tissue section of only 5 mm(2) and 10 mu m thickness. Furthermore, spatially resolved proteome distribution profiles of enterocytes, lymphocytes, and smooth muscle cells on the same tissue slices and across four consecutive sections with micrometer distance were successfully achieved. This fully integrated proteomics technology, termed LCM-SISPROT, is therefore promising for spatial-resolution cell type proteome profiling of tumor microenvironment with a minute amount of clinical starting materials.

SCI ; EI

英语

NI期刊

通讯

Shenzhen Health and Family Planning Commission[SZXJ2017025]

Chemistry

Chemistry, Analytical

WOS:000431464400050

AMER CHEMICAL SOC

20181905166432

Diseases ; Image resolution ; Lymphocytes ; Microdissection ; Molecular biology ; Muscle ; Proteins ; Tumors

Bioengineering and Biology:461 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen 518020, Peoples R China
2.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
3.Jinan Univ, Sch Basic Med, Dept Pathophysiol, Guangzhou 510632, Guangdong, Peoples R China
4.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou 510632, Guangdong, Peoples R China
5.Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen 518055, Peoples R China

Xu, Ruilian,Tang, Jun,Deng, Quantong,et al. Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology[J]. ANALYTICAL CHEMISTRY,2018,90(9):5879-5886.

Xu, Ruilian.,Tang, Jun.,Deng, Quantong.,He, Wan.,Sun, Xiujie.,...&Tian, Ruijun.(2018).Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology.ANALYTICAL CHEMISTRY,90(9),5879-5886.

Xu, Ruilian,et al."Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology".ANALYTICAL CHEMISTRY 90.9(2018):5879-5886.