Purification of Semiconducting Polymer Dots by Size Exclusion Chromatography Prior to Cytotoxicity Assay and Stem Cell Labeling

Chen, Dandan1; Yuan, Ye2; Yu, Jiangbo3; Chiu, Daniel T.3; Wu, Changfeng1

2018-05

10.1021/aca.analchem.8b00095

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Semiconducting polymer dots (Pdots) as fluorescent probes have shown promising applications because of their excellent optical properties. However, apparent differences were observed in cytotoxicity assays, which might originate from impurities introduced in polymer synthesis or nanoparticle preparation. A simple gel-filtration-based purification method was used to address this issue. Purified Pdots displayed obviously decreased cytotoxicity as compared with the same batch of unpurified Pdots. The purified Pdots were further examined in a cytotoxicity study on mesenchymal stem cells (MSCs), which are very sensitive to exogenous probes. The results indicated that purified Pdots did not affect the proliferation ability of MSCs, while unpurified Pdots could have obvious cytotoxicity. In addition, the purified Pdots did not show cytotoxicity even after 6 months of storage. Our results demonstrated that gel filtration is an effective method for obtaining Pdots with minimal cytotoxicity, which are more suitable for biological applications.

SCI ; EI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Commission[JCYJ20170307110157501]

Chemistry

Chemistry, Analytical

WOS:000431464400011

AMER CHEMICAL SOC

20181905148825

Assays ; Cell culture ; Cytotoxicity ; Flowcharting ; Optical properties ; Polymers ; Probes ; Purification ; Stem cells ; Synthesis (chemical)

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Computer Programming:723.1 ; Light/Optics:741.1 ; Chemistry:801 ; Chemical Reactions:802.2 ; Polymeric Materials:815.1

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 510855, Guangdong, Peoples R China
2.Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China
3.Univ Washington, Dept Chem, Seattle, WA 98195 USA

Chen, Dandan,Yuan, Ye,Yu, Jiangbo,et al. Purification of Semiconducting Polymer Dots by Size Exclusion Chromatography Prior to Cytotoxicity Assay and Stem Cell Labeling[J]. ANALYTICAL CHEMISTRY,2018,90(9):5569-5575.

Chen, Dandan,Yuan, Ye,Yu, Jiangbo,Chiu, Daniel T.,&Wu, Changfeng.(2018).Purification of Semiconducting Polymer Dots by Size Exclusion Chromatography Prior to Cytotoxicity Assay and Stem Cell Labeling.ANALYTICAL CHEMISTRY,90(9),5569-5575.

Chen, Dandan,et al."Purification of Semiconducting Polymer Dots by Size Exclusion Chromatography Prior to Cytotoxicity Assay and Stem Cell Labeling".ANALYTICAL CHEMISTRY 90.9(2018):5569-5575.