A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells

Yu, Jiantao; He, Sihui; Shao, Chen; Zhao, Haoran; Li, Jing; Tian, Leilei

2018-04-21

10.1039/c8nr00364e

Nanoscale   影响因子和分区

2040-3364

2040-3372

The design of a nano-system for the detection of intracellular microRNAs is challenging as it must fulfill complex requirements, i.e., it must have a high sensitivity to determine the dynamic expression level, a good reliability for multiplex and simultaneous detection, and a satisfactory biostability to work in biological environments. Instead of employing a commonly used physisorption or a full-conjugation strategy, here, a GO-DNA nano-system was developed under graft/base-pairing construction. The common anchor sequence was chemically grafted to GO to base-pair with various microRNA probes; and the hybridization with miRNAs drives the dyes on the probes to leave away from GO, resulting in "turned-on" fluorescence. This strategy not only simplifies the synthesis but also efficiently balances the loading yields of different probes. Moreover, the conjugation yield of GO with a base-paired hybrid has been improved by more than two-fold compared to that of the conjugation with a single strand. We demonstrated that base-paired DNA probes could be efficiently delivered into cells along with GO and are properly stabilized by the conjugated anchor sequence. The resultant GO-DNA nano-system exhibited high stability in a complex biological environment and good resistance to nucleases, and was able to accurately discriminate various miRNAs without cross-reaction. With all of these positive features, the GO-DNA nano-system can simultaneously detect three miRNAs and monitor their dynamic expression levels.

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China[51503096]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000430537200035

ROYAL SOC CHEMISTRY

20181705057559

DNA ; Grafting (chemical) ; RNA

Biological Materials and Tissue Engineering:461.2 ; Nanotechnology:761 ; Chemical Reactions:802.2 ; Solid State Physics:933

Web of Science

Southern Univ Sci & Technol, Dept Mat Sci & Engn, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China

Yu, Jiantao,He, Sihui,Shao, Chen,et al. A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells[J]. Nanoscale,2018,10(15):7067-7076.

Yu, Jiantao,He, Sihui,Shao, Chen,Zhao, Haoran,Li, Jing,&Tian, Leilei.(2018).A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells.Nanoscale,10(15),7067-7076.

Yu, Jiantao,et al."A common anchor facilitated GO-DNA nano-system for multiplex microRNA analysis in live cells".Nanoscale 10.15(2018):7067-7076.