| 题名 | Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32 |
| 作者 | |
| 通讯作者 | Sun, Shan; Sui, Sen-Fang |
| 发表日期 | 2022
|
| DOI | |
| 发表期刊 | |
| ISSN | 2375-2548
|
| 卷号 | 8期号:4 |
| 摘要 | Transport protein particle (TRAPP) complexes belong to the multiprotein tethering complex and exist in three forms-core TRAPP/TRAPPI, TRAPPII, and TRAPPIII. TRAPPII activates GTPase Ypt31/Ypt32 as the guanine nucleotide exchange factor in the trans-Golgi network to determine the maturation of Golgi cisternae into post-Golgi carriers in yeast. Here, we present cryo-EM structures of yeast TRAPPII in apo and Ypt32-bound states. All the structures show a dimeric architecture assembled by two triangle-shaped monomers, while the monomer in the apo state exhibits both open and closed conformations, and the monomer in the Ypt32-bound form only captures the closed conformation. Located in the interior of the monomer, Ypt32 binds with both core TRAPP/TRAPPI and Trs120 via its nucleotide-binding domain and binds with Trs31 via its hypervariable domain. Combined with functional analysis, the structures provide insights into the assembly of TRAPPII and the mechanism of the specific activation of Ypt31/Ypt32 by TRAPPII. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
|
| 重要成果 | NI论文
|
| 学校署名 | 通讯
|
| 资助项目 | National Natural Science Foundation of China[31670745,31861143048,31670746]
; National Basic Research Program["2017YFA0504600","2016YFA0501101"]
|
| WOS研究方向 | Science & Technology - Other Topics
|
| WOS类目 | Multidisciplinary Sciences
|
| WOS记录号 | WOS:000747329300004
|
| 出版者 | |
| EI入藏号 | 20220511555076
|
| EI主题词 | Activation analysis
; Chemical activation
; Complex networks
; Conformations
; Medical imaging
; Nucleotides
; Yeast
|
| EI分类号 | Biomedical Engineering:461.1
; Biology:461.9
; Computer Systems and Equipment:722
; Imaging Techniques:746
; Physical Chemistry:801.4
; Chemical Reactions:802.2
; Chemical Products Generally:804
; Food Products:822.3
|
| 来源库 | Web of Science
|
| 引用统计 |
被引频次[WOS]：11
|
| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/278878 |
| 专题 | 生命科学学院_生物系 |
| 作者单位 | 1.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Beijing Frontier Res Ctr Biol Struct, Sch Life Sci,State Key Lab Membrane Biol, Beijing 100084, Peoples R China 2.Natl Inst Biol Sci, Beijing 102206, Peoples R China 3.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China |
| 通讯作者单位 | 生物系 |
| 推荐引用方式 GB/T 7714 |
Mi, Chenchen,Zhang, Li,Huang, Guoqiang,et al. Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32[J]. Science Advances,2022,8(4).
|
| APA |
Mi, Chenchen.,Zhang, Li.,Huang, Guoqiang.,Shao, Guangcan.,Yang, Fan.,...&Sui, Sen-Fang.(2022).Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32.Science Advances,8(4).
|
| MLA |
Mi, Chenchen,et al."Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32".Science Advances 8.4(2022).
|
| 条目包含的文件 | 条目无相关文件。 | |||||
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