Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation

Deng, Yu1; Wang, Yulin1; Mao, Yanping1,2; Zhang, Tong1,3

2018-03-06

10.1021/acs.est.7b05913

ENVIRONMENTAL SCIENCE & TECHNOLOGY   影响因子和分区

0013-936X

1520-5851

In this study, metagenomic analyses were combined with cultivation-based techniques as a nested approach to identify functionally significant bacteria for sulfadiazine biodegradation within enrichment communities. The metagenomic investigations indicated that our previously isolated sulfadiazine degrader, Arthrobacter sp. D2, and another Pimelobacter bacterium concomitantly occurred as most abundant members in the community of an enrichment culture that performed complete sulfadiazine mineralization for over two years. Responses of the enriched populations to sole carbon source alternation further suggested the ability of this Pimelobacter member to grow on 2-aminopyrimidine, the most prominent intermediate metabolite of sulfadiazine. Taking advantage of this propensity, additional cultivation procedures have enabled the successful isolation of Pimelobacter sp. LG209, whose genomic sequences exactly matched that of the dominant Pimelobacter bacterium in the sulfadiazine enrichment culture. Integration of metagenomic investigations with the physiological characteristics of the isolates conclusively demonstrated that the sulfadiazine mineralization in a long-running enrichment culture was prominently mediated by primary sulfadiazine-degrading specialist strain Arthrobacter sp. D2 in association with the 2-aminopyrimidine-degrading partner strain Pimelobacter sp. LG209. Here, we provided the first mechanistic insight into microbial interactions in steady sulfadiazine mineralization processes, which will help develop appropriate bioremediation strategies for sulfadiazine-contaminated hotspot sites.

SCI ; EI

英语

NI期刊

通讯

Shenzhen Science & Technology Project Program[JCYJ20160520165135743]

Engineering ; Environmental Sciences & Ecology

Engineering, Environmental ; Environmental Sciences

WOS:000427202700061

AMER CHEMICAL SOC

20181104900562

Bacteria ; Biodegradation ; Bioremediation

Environmental Impact and Protection:454.2 ; Biotechnology:461.8 ; Mineralogy:482

ENVIRONMENT/ECOLOGY

Web of Science

1.Univ Hong Kong, Dept Civil Engn, Environm Biotechnol Lab, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China
2.Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China
3.Southern Univ Sci & Technol, Int Ctr Antibiot Resistance Environm, Shenzhen 518055, Peoples R China

Deng, Yu,Wang, Yulin,Mao, Yanping,et al. Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2018,52(5):2963-2972.

Deng, Yu,Wang, Yulin,Mao, Yanping,&Zhang, Tong.(2018).Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation.ENVIRONMENTAL SCIENCE & TECHNOLOGY,52(5),2963-2972.

Deng, Yu,et al."Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation".ENVIRONMENTAL SCIENCE & TECHNOLOGY 52.5(2018):2963-2972.