Capturing the interactome of newly transcribed RNA

Bao, Xichen1,2,3,4; Guo, Xiangpeng1,2,3,4; Yin, Menghui5; Tariq, Muqddas1,2,3,4,6; Lai, Yiwei1,2,3,4,6; Kanwal, Shahzina1,2,3,4; Zhou, Jiajian7; Li, Na1,2,3,4,8; Lv, Yuan1,2,3,4,6; Pulido-Quetglas, Carlos9; Wang, Xiwei1,2,3,4; Ji, Lu7; Khan, Muhammad J.1,2,3,4,10; Zhu, Xihua1,2,3,4; Luo, Zhiwei1,2,3,4,6; Shao, Changwei11; Lim, Do-Hwan11; Liu, Xiao12; Li, Nan13; Wang, Wei14; He, Minghui15; Liu, Yu-Lin16; Ward, Carl2,3,4; Wang, Tong17; Zhang, Gong17; Wang, Dongye1,2,3,4,18; Yang, Jianhua19; Chen, Yiwen20; Zhang, Chaolin21; Jauch, Ralf18; Yang, Yun-Gui22; Wang, Yangming23; Qin, Baoming1,2; Anko, Minna-Liisa24; Hutchins, Andrew P.25; Sun, Hao7; Wang, Huating7; Fu, Xiang-Dong11; Zhang, Biliang5; Esteban, Miguel A.1,2,3,4

2018-03

10.1038/NMETH.4595

NATURE METHODS   影响因子和分区

1548-7091

1548-7105

We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RIC K will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.

SCI

英语

其他

[91440110]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000426303200018

NATURE PUBLISHING GROUP

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Key Lab Regenerat Biol, Joint Sch Life Sci, Guangzhou, Guangdong, Peoples R China
2.Guangzhou Med Univ, Guangzhou, Guangdong, Peoples R China
3.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Lab RNA Chromatin & Human Dis, Key Lab Regenerat Biol, Guangzhou, Guangdong, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cells & Regenerat Med, Guangzhou, Guangdong, Peoples R China
5.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Lab RNA Chem Biol, State Key Lab Resp Dis, Guangzhou, Guangdong, Peoples R China
6.Univ Chinese Acad Sci, Beijing, Peoples R China
7.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Prince Wales Hosp, Dept Orthopaed & Traumatol, Hong Kong, Hong Kong, Peoples R China
8.Anhui Univ, Inst Hlth Sci, Hefei, Anhui, Peoples R China
9.Univ Bern, Dept Clin Res, Bern, Switzerland
10.COMSATS Inst Informat Technol, Dept Biosci, Pk Rd, Islamabad, Pakistan
11.Univ Calif San Diego, Dept Cellular & Mol Med, San Diego, CA 92103 USA
12.GFK Biotech Inc, Dept Bioinformat, Shanghai, Peoples R China
13.Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Synthet Biol Engn Res, Shenzhen, Guangdong, Peoples R China
14.Guangzhou RiboBio Co Ltd, Guangzhou, Guangdong, Peoples R China
15.Forevergen Biosci Ctr, Guangzhou, Guangdong, Peoples R China
16.FitGene BioTechnol Co Ltd, Guangzhou, Guangdong, Peoples R China
17.Jinan Univ, Key Lab Funct Prot Res Guangdong Higher Educ Inst, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China
18.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Drug Discovery Pipeline Grp, Guangzhou, Guangdong, Peoples R China
19.Sun Yat Sen Univ, Key Lab Gene Engn, State Key Lab Biocontrol, Minist Educ, Guangzhou, Guangdong, Peoples R China
20.Univ Texas MD Anderson Canc Ctr, Dept Bioinformat & Computat Biol, Houston, TX 77030 USA
21.Columbia Univ, Dept Biochem & Mol Biophys, Dept Syst Biol, Dept Ctr Motor Neuron Biol & Dis, New York, NY USA
22.Chinese Acad Sci, Beijing Inst Genom, Collaborat Innovat Ctr Genet & Dev, Ctr Excellence Mol Cell Sci,Key Lab Genom & Preci, Beijing, Peoples R China
23.Peking Univ, Beijing Key Lab Cardiometab Mol Med, Inst Mol Med, Beijing, Peoples R China
24.Monash Univ, Dept Anat & Dev Biol & Biomed Discovery Inst, Melbourne, Vic, Australia
25.Southern Univ Sci & Technol China, Dept Biol, Shenzhen, Peoples R China

Bao, Xichen,Guo, Xiangpeng,Yin, Menghui,et al. Capturing the interactome of newly transcribed RNA[J]. NATURE METHODS,2018,15(3):213-+.

Bao, Xichen.,Guo, Xiangpeng.,Yin, Menghui.,Tariq, Muqddas.,Lai, Yiwei.,...&Esteban, Miguel A..(2018).Capturing the interactome of newly transcribed RNA.NATURE METHODS,15(3),213-+.

Bao, Xichen,et al."Capturing the interactome of newly transcribed RNA".NATURE METHODS 15.3(2018):213-+.