Large-field-of-view optical resolution photoacoustic microscopy

Qin, Wei1,2; Jin, Tian1,2; Guo, Heng1,2; Xi, Lei1,2

2018-02-19

10.1364/OE.26.004271

OPTICS EXPRESS   影响因子和分区

1094-4087

The use of existing optical resolution photoacoustic microscopy (ORPAM) has been limited to small organs or part of large organs due to the millimeter-scale field of view (FOV) in both lateral and axial directions. Here, we report a large-field-of-view ORPAM (L-ORPAM) using a combination of a new scanning mechanism and an ultrafast pulsed laser. Phantom and in vivo experiments show that L-ORPAM has a spatial FOV of 40 mm in lateral and 12 mm in axial, which expends the effective imaging domain to one order that of existing ORPAMs. To show the advantages of L-ORPAM, we apply it to imaging vasculatures of both brain and ears simultaneously in mice, and to visualizing intestinal vasculatures in rats. The result suggests that L-ORPAM has sufficient contrast, resolution and spatial FOV to carry out studies of large rodents. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

SCI ; EI

英语

第一 ; 通讯

State International Collaboration Program from Sichuan[2016HH0019]

Optics

Optics

WOS:000426268500048

OPTICAL SOC AMER

20180804817857

Mammals

Imaging Techniques:746

PHYSICS

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Elect Sci & Technol China, Sch Phys Elect, Chengdu 610054, Sichuan, Peoples R China

Qin, Wei,Jin, Tian,Guo, Heng,et al. Large-field-of-view optical resolution photoacoustic microscopy[J]. OPTICS EXPRESS,2018,26(4):4271-4278.

Qin, Wei,Jin, Tian,Guo, Heng,&Xi, Lei.(2018).Large-field-of-view optical resolution photoacoustic microscopy.OPTICS EXPRESS,26(4),4271-4278.

Qin, Wei,et al."Large-field-of-view optical resolution photoacoustic microscopy".OPTICS EXPRESS 26.4(2018):4271-4278.