Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR-Cas9 and ssODN in human cancer cells

Hu, Zheng1,2; Shi, Zhaoying3; Guo, Xiaogang4; Jiang, Baishan5; Wang, Guo1; Luo, Dixian2; Chen, Yonglong3; Zhu, Yuan-Shan1,6

2018-02-19

10.1186/s13578-018-0200-z

Cell and Bioscience   影响因子和分区

2045-3701

Background: Precise genome editing is essential for both basic and translational research. The recently developed CRISPR/Cas9 system can specifically cleave a designated site of target gene to create a DNA double-strand break, which triggers cellular DNA repair mechanism of either inaccurate non-homologous end joining, or site-specific homologous recombination. Unfortunately, homology-directed repair (HDR) is challenging due to its very low efficiency. Herein, we focused on improving the efficiency of HDR using a combination of CRISPR/Cas9, eGFP, DNA ligase IV inhibitor SCR7, and single-stranded oligodeoxynucleotides (ssODN) in human cancer cells.

CRISPR/Cas9 Homology-directed repair DNA ligase IV inhibitor Single-stranded oligodeoxynucleotides (ssODN) Non-homologous DNA end joining

SCI

英语

通讯

Shenzhen Key Laboratory of Cell Microenvironment[ZDSYS20140509142721429]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:000425456300001

BIOMED CENTRAL LTD

Web of Science

1.Cent S Univ, Xiangya Hosp, Dept Clin Pharmacol, Changsha 410078, Hunan, Peoples R China
2.First Peoples Hosp Chenzhou, Translat Med Inst, Natl & Local Joint Engn Lab High Mol Diag Technol, Chenzhou 432000, Hunan, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Key Lab Cell Microenvironm, Dept Biol, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen 518055, Guangdong, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China
5.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Inst Chem Biol, Guangzhou 510530, Guangdong, Peoples R China
6.Weill Cornell Med Coll, Dept Med, New York, NY 10065 USA

Hu, Zheng,Shi, Zhaoying,Guo, Xiaogang,et al. Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR-Cas9 and ssODN in human cancer cells[J]. Cell and Bioscience,2018,8.

Hu, Zheng.,Shi, Zhaoying.,Guo, Xiaogang.,Jiang, Baishan.,Wang, Guo.,...&Zhu, Yuan-Shan.(2018).Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR-Cas9 and ssODN in human cancer cells.Cell and Bioscience,8.

Hu, Zheng,et al."Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR-Cas9 and ssODN in human cancer cells".Cell and Bioscience 8(2018).