Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies

Wu, Shanshan1,2; Wu, Siying1; Yi, Zheyuan1; Zeng, Fei1; Wu, Weizhen1; Qiao, Yuan1; Zhao, Xingzhong2; Cheng, Xing1; Tian, Yanqing1

2018-02

10.3390/s18020564

SENSORS   影响因子和分区

1424-8220

In this study, we developed fluorescent dual pH and oxygen sensors loaded in multi-well plates for in-situ and high-throughput monitoring of oxygen respiration and extracellular acidification during microbial cell growth for understanding metabolism. Biocompatible PHEMA-co-PAM materials were used as the hydrogel matrix. A polymerizable oxygen probe (OS2) derived from PtTFPP and a polymerizable pH probe (S2) derived from fluorescein were chemically conjugated into the matrix to solve the problem of the probe leaching from the matrix. Gels were allowed to cure directly on the bottom of 96-well plates at room-temperature via redox polymerization. The influence of matrix's composition on the sensing behaviors was investigated to optimize hydrogels with enough robustness for repeatable use with good sensitivity. Responses of the dual sensing hydrogels to dissolved oxygen (DO) and pH were studied. These dual oxygen-pH sensing plates were successfully used for microbial cell-based screening assays, which are based on the measurement of fluorescence intensity changes induced by cellular oxygen consumption and pH changes during microbial growth. This method may provide a real-time monitoring of cellular respiration, acidification, and a rapid kinetic assessment of multiple samples for cell viability as well as high-throughput drug screening. All of these assays can be carried out by a conventional plate reader.

dual oxygen-pH sensing plates oxygen respiration extracellular acidification high throughput analysis

SCI ; EI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Committee for key laboratory[ZDSYS20140509142721431]

Chemistry ; Electrochemistry ; Instruments & Instrumentation

Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation

WOS:000427544000247

MDPI

20180804818819

Acidification ; Biocompatibility ; Cells ; Cytology ; Dyes ; Fluorescence ; Metabolism ; Oxygen sensors ; pH sensors ; PHEMA ; Physiology ; Platinum compounds ; Probes ; Screening ; Throughput

Bioengineering and Biology:461 ; Light/Optics:741.1 ; Chemistry, General:801.1 ; Chemical Operations:802.3 ; Chemical Agents and Basic Industrial Chemicals:803 ; Polymeric Materials:815.1 ; Food Processing Operations:822.2

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, 1088 Xueyuan Rd, Shenzhen 518055, Peoples R China
2.Wuhan Univ, Dept Phys, Wuhan 430072, Hubei, Peoples R China

Wu, Shanshan,Wu, Siying,Yi, Zheyuan,et al. Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies[J]. SENSORS,2018,18(2).

Wu, Shanshan.,Wu, Siying.,Yi, Zheyuan.,Zeng, Fei.,Wu, Weizhen.,...&Tian, Yanqing.(2018).Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies.SENSORS,18(2).

Wu, Shanshan,et al."Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies".SENSORS 18.2(2018).