The COP9 Signalosome regulates seed germination by facilitating protein degradation of RGL2 and ABI5

Jin, Dan1,4; Wu, Ming1; Li, Bosheng1,5; Bucker, Birte1,6; Keil, Philipp1,6; Zhang, Shaoman2,3; Li, Jigang2; Kang, Dingming3; Liu, Jie1; Dong, Jie1; Deng, Xing Wang1,7,8; Irish, Vivian1; Wei, Ning1

2018-02

10.1371/journal.pgen.1007237

PLOS GENETICS   影响因子和分区

1553-7404

The control of seed germination and seed dormancy are critical for the successful propagation of plant species, and are important agricultural traits. Seed germination is tightly controlled by the balance of gibberellin (GA) and abscisic acid (ABA), and is influenced by environmental factors. The COP9 Signalosome (CSN) is a conserved multi-subunit protein complex that is best known as a regulator of the Cullin-RING family of ubiquitin E3 ligases (CRLs). Multiple viable mutants of the CSN showed poor germination, except for csn5b-1. Detailed analyses showed that csn1-10 has a stronger seed dormancy, while csn5a-1 mutants exhibit retarded seed germination in addition to hyperdormancy. Both csn5a-1 and csn1-10 plants show defects in the timely removal of the germination inhibitors: RGL2, a repressor of GA signaling, and ABI5, an effector of ABA responses. We provide genetic evidence to demonstrate that the germination phenotype of csn1-10 is caused by over-accumulation of RGL2, a substrate of the SCF (CRL1) ubiquitin E3 ligase, while the csn5a-1 phenotype is caused by over-accumulation of RGL2 as well as ABI5. The genetic data are consistent with the hypothesis that CSN5A regulates ABI5 by a mechanism that may not involve CSN1. Transcriptome analyses suggest that CSN1 has a more prominent role than CSN5A during seed maturation, but CSN5A plays a more important role than CSN1 during seed germination, further supporting the functional distinction of these two CSN genes. Our study delineates the molecular targets of the CSN complex in seed germination, and reveals that CSN5 has additional functions in regulating ABI5, thus the ABA signaling pathway.

SCI

英语

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其他

Fundamental Research Funds for the Central Universities from China[XDJK2017B001]

Genetics & Heredity

Genetics & Heredity

WOS:000426361900035

PUBLIC LIBRARY SCIENCE

Web of Science

1.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China
3.China Agr Univ, MOE Key Lab Crop Heterosis & Utilizat, Coll Agron & Biotechnol, Beijing, Peoples R China
4.Southwest Univ, Biotechnol Res Ctr, Minist Agr, Key Lab Biotechnol & Crop Qual Improvement, Chongqing, Peoples R China
5.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China
6.Justus Liebig Univ Giessen, Inst Biochem, Giessen, Germany
7.Peking Univ, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing, Peoples R China
8.Peking Univ, Sch Life Sci, Beijing, Peoples R China

Jin, Dan,Wu, Ming,Li, Bosheng,et al. The COP9 Signalosome regulates seed germination by facilitating protein degradation of RGL2 and ABI5[J]. PLOS GENETICS,2018,14(2).

Jin, Dan.,Wu, Ming.,Li, Bosheng.,Bucker, Birte.,Keil, Philipp.,...&Wei, Ning.(2018).The COP9 Signalosome regulates seed germination by facilitating protein degradation of RGL2 and ABI5.PLOS GENETICS,14(2).

Jin, Dan,et al."The COP9 Signalosome regulates seed germination by facilitating protein degradation of RGL2 and ABI5".PLOS GENETICS 14.2(2018).