Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging

Su, Fengyu1; Agarwal, Shubhangi2; Pan, Tingting3,4; Qiao, Yuan3; Zhang, Liqiang1; Shi, Zhengwei1; Kong, Xiangxing1; Day, Kevin1; Chen, Meiwan4; Meldrum, Deirdre1; Kodibagkar, Vikram D.2; Tian, Yanqing3

2018-01-17

10.1021/acsami.7b15796

ACS Applied Materials & Interfaces   影响因子和分区

1944-8244

1944-8252

In this paper, we report synthesis and characterization of a novel multimodality (MRI/fluorescence) probe for pH sensing and imaging. A multifunctional polymer was derived from poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) and integrated with a naphthalimide-based-ratiometric fluorescence probe and a gadolinium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complex (Gd-DOTA complex). The polymer was characterized using UV-vis absorption spectrophotometry, fluorescence spectrofluorophotometry, magnetic resonance imaging (MRI), and confocal microscopy for optical and MRI-based pH sensing and cellular imaging. In vitro labeling of macrophage J774 and esophageal CP-A cell lines shows the polymer's ability to be internalized in the cells. The transverse relaxation time (T-2) of the polymer was observed to be pH-dependent, whereas the spin-lattice relaxation time (T-1) was not. The pH probe in the polymer shows a strong fluorescence-based ratiometric pH response with emission window changes, exhibiting blue emission under acidic conditions and green emission under basic conditions, respectively. This study provides new materials with multimodalities for pH sensing and imaging.

polymer pH sensor ratiometric sensor MRI fluorescence imaging dual-modality

SCI ; EI

英语

通讯

Southern University of Science and Technology[2015-FRG-SUSTC1501A-01] ; Southern University of Science and Technology[25/Y01256009] ; Southern University of Science and Technology[25/Y01256114] ; Southern University of Science and Technology[25/Y01256214]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000423140400012

AMER CHEMICAL SOC

20180404670119

Cell culture ; Gadolinium compounds ; Magnetic resonance imaging ; pH ; pH sensors ; Polymers ; Probes ; Relaxation time

Imaging Techniques:746 ; Chemistry, General:801.1 ; Polymeric Materials:815.1 ; Classical Physics; Quantum Theory; Relativity:931

Web of Science

1.Arizona State Univ, Biodesign Inst, Ctr Biosignatures Discovery Automat, Tempe, AZ 85287 USA
2.Arizona State Univ, Sch Biol & Hlth Syst Engn, Tempe, AZ 85281 USA
3.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China
4.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Ave Univ, Taipa 999078, Macau, Peoples R China

Su, Fengyu,Agarwal, Shubhangi,Pan, Tingting,et al. Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging[J]. ACS Applied Materials & Interfaces,2018,10(2):1556-1565.

Su, Fengyu.,Agarwal, Shubhangi.,Pan, Tingting.,Qiao, Yuan.,Zhang, Liqiang.,...&Tian, Yanqing.(2018).Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging.ACS Applied Materials & Interfaces,10(2),1556-1565.

Su, Fengyu,et al."Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging".ACS Applied Materials & Interfaces 10.2(2018):1556-1565.